Folic Acid in Intrauterine Growth Retarded Early Weaner Piglets: Effects on Hepatic Structure and Apoptosis-Related Gene Expression

YAO Ying, CHEN Daiwen, Liu Jingbo, MAO Xiangbing, MAO Qian, YU Bing

doi:10.3969/j.issn.1006-267x.2012.02.012

2012 , Vol. 24 >Issue 2: 271 - 279

DOI: https://doi.org/10.3969/j.issn.1006-267x.2012.02.012

Molecular Nutrition

Folic Acid in Intrauterine Growth Retarded Early Weaner Piglets: Effects on Hepatic Structure and Apoptosis-Related Gene Expression

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Key Laboratory of Sichuan Province, Animal Disease-Resistance Nutrition Institute of Animal Nutrition, Sichuan Agricultural University, Ya’an 625014, China

Received date: 2011-08-11

Online published: 2012-02-07

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Abstract

This experiment was conducted to study the effects of dietary folic acid level on hepatic structure and apoptosis-related gene expression in early weaner piglets suffered intrauterine growth retardation (IUGR). Twenty four crossbred (Duroc×Landrace×Yorkshire) male piglets, weaned at 14 days of age, with an average body weight of (2.79±0.34) kg were randomly assigned into 3 groups with 8 replicates in each group and 1 piglet per replicate. Piglets were fed diets supplemented with 0, 5 and 10 mg/kg folic acid, respectively. The experiment lasted for 21 d. The results showed as follows: 1) dietary supplementation of 5 (P<0.05) and 10 mg/kg (P<0.01) folic acid significantly decreased serum triglycerides concentration of piglets at 35 days of age, but had no significant effect on serum glucose concentration (P>0.05). 2) Dietary supplementation of 5 mg/kg folic acid significantly decreased hepatocyte diameter (P<0.05). 3) Dietary supplementation of 5 mg/kg folic acid significantly up-regulated gene expression of Bcl-2 (P<0.05), but significantly down-regulated gene expression of Bax and p53 (P<0.01); however, gene expression of Bax (P<0.01) and p53 (P<0.05) was significantly reduced by dietary supplementation of 10 mg/kg folic acid. 4) Expression of ATM and APE-1 as well as key enzyme encoding genes involved in one-carbon metabolism was not affected by folic acid supplementation. These results indicate that supplementation of folic acid at proper level can improve hepatic structure and apoptosis-related gene (Bcl-2, Bax and p53) expression of IUGR piglets; under conditions in this experiment, the optimum level is 5 mg/kg.

Key words： folic acid; intrauterine growth retardation; hepatic structure; apoptosis; one-carbon unit; early weaner piglets

Cite this article

YAO Ying, CHEN Daiwen, Liu Jingbo, MAO Xiangbing, MAO Qian, YU Bing . Folic Acid in Intrauterine Growth Retarded Early Weaner Piglets: Effects on Hepatic Structure and Apoptosis-Related Gene Expression[J]. Chinese Journal of Animal Nutrition, 2012 , 24(2) : 271 -279 . DOI: 10.3969/j.issn.1006-267x.2012.02.012

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