The experiment was conducted to study the effects of dietary glutamine (Gln) on small intestinal structure and absorption in broilers suffered from heat stress. Two hundred and forty Cobb-500 one-day-old broilers were randomly selected and divided into 6 treatments with 4 replicates in each treatment and 10 chicks in each replicate. The broilers in group Ⅰ(control group) were fed a basal diet, and those in groups Ⅱ, Ⅲ, Ⅳ, Ⅴ and Ⅵ were fed the basal diet supplemented with 0.4%, 0.8%, 1.2%, 1.6% and 2.0% glutamine, respectively. The experiment lasted for four weeks (days 15 to 42). During the experimental period, the temperature was maintained at (35±2) ℃ from 09:00 to 17:00, and was maintained at (30±2) ℃ from 17:00 to 09:00 of the next day. The relative humidity of the chicken house was controlled between 70% and 80%. The results showed as follows: 1) the supplementation of Gln in diets significantly influenced growth performance of broilers aged from 29 to 42 days (P<0.05), in which the supplementation of 1.2% Gln was the best, but there was no significant difference among all groups during 15 to 28 days (P>0.05). 2) At 28 days of age, the supplementation of Gln from 0.8% to 2.0% in diets significantly increased the villus height of duodenum and jejunum of broilers (P<0.05 or P<0.01), and 2.0% Gln group was significantly higher than the other groups (P<0.01). The supplementation of 0.4%, 1.2% and 2.0% Gln in diets significantly increased the villus height of ileum of broilers (P<0.05 or P<0.01), and 1.2% Gln group was significantly higher than the other groups (P<0.01). At 42 days of age, the supplementation of Gln in diets significantly decreased the villus width and crypt depth of duodenum of broilers (P<0.05 or P<0.01); the supplementation of 1.2% Gln in diets significantly increased the villus height of duodenum (P<0.01); the supplementation of 1.2%, 1.6% and 2.0% Gln in diets significantly increased the villus height and decreased the villus width (P<0.05 or P<0.01); and the supplementation of 1.2% Gln in diets significantly decreased the thickness of lamina propria of jejunum and ileum (P<0.05 or P<0.01). 3) The small intestinal absorption was significantly increased when the supplemental level of Gln was 2.0% at 28 days of age (P<0.01) and 1.2% at 42 days of age (P<0.05). In conclusion, dietary glutamine can improve the small intestinal structure and absorption in broilers suffered from heat stress, and the suitable level in diets is 2.0% from 15 to 28 days of age and 1.2% from 29 to 42 days of age.
LU Jing, LI Wenli, JIANG Jianyang, LI Fangzheng, REN Huiying
. Dietary Glutamine Affects Small Intestinal Structure and Absorption in Broilers[J]. Chinese Journal of Animal Nutrition, 2012
, 24(2)
: 291
-300
.
DOI: 10.3969/j.issn.1006-267x.2012.02.015
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