This experiment was conducted to study the effects of dietary nutrient levels on reproductive performance, serum biochemical indices and reproductive hormones of pregnant and lactating Rex rabbits. One hundred multiparous female Rex rabbits with an average body weight of (4.73±0.42) kg were randomly assigned to 5 groups with 20 replicates in each group, and each replicate contained 1 rabbit. Rex rabbits in the 5 groups were fed 5 experimental diets containing 10.5 MJ/kg DE+18% CP, 10.5 MJ/kg DE+16% CP, 10.0 MJ/kg DE+17% CP, 9.5 MJ/kg DE+18% CP, 9.5 MJ/kg DE+16% CP, respectively. The experiment lasted from mating of female rabbits to baby rabbits at 45 days of age. The results showed as follows: dietary nutrient levels significantly affected weaning litter weight (P=0.036 5), but did not significantly affect the other reproductive performance indices (P>0.05). The dietary nutrient levels had significant influence on glutamic-oxaloacetic transaminase activity (P=0.043 9) and contents of cholesterol (P=0.047 8), high density lipoprotein cholesterol (P=0.025 6) and low density lipoprotein cholesterol (P=0.024 8) in serum of pregnant Rex rabbits, but had no significant influence on serum reproductive hormones of pregnant Rex rabbits (P>0.05). The contents of total protein (P=0.036 9), urea nitrogen (P=0.047 3) and cholesterol (P=0.023 2) in serum of lactating Rex rabbits were significantly affected by dietary nutrient levels. The dietary nutrient levels had no significant influence on serum reproductive hormones (except leuteinizing hormone) of lactating Rex rabbits (P>0.05). In conclusion, when the dietary DE and CP levels are varied from 9.5 to 10.5 MJ/kg and 16% to 18%, respectively, the dietary nutrient levels have no influence on reproductive performance and serum reproductive hormones, but have influence on partial serum biochemical indices of pregnant and lactating Rex rabbits.