Effects of Dietary Protein Level on Gastrointestinal Digestive Enzyme Activity and Jejunal Morphology in Minks during Winter Hair Period

ZHANG Tietao, CUI Hu, YUE Zhigang, YANG Ying, GAO Xiuhua, YANG Fuhe, XING Xiumei

doi:10.3969/j.issn.1006-267x.2012.02.027

2012 , Vol. 24 >Issue 2: 376 - 382

DOI: https://doi.org/10.3969/j.issn.1006-267x.2012.02.027

Short Communications

Effects of Dietary Protein Level on Gastrointestinal Digestive Enzyme Activity and Jejunal Morphology in Minks during Winter Hair Period

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1. Institute of Feed Research, Chinese Academy of Agricultural Sciences, Beijing 100081, China;
2. State Key Laboratory of Special Economic Animal Molecular Biology, Jilin 132109, China;
3. Institute of Special Economic Animal and Plant Science, Chinese Academy of Agricultural Sciences, Jilin 132109, China

Received date: 2011-08-20

Online published: 2012-02-07

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Abstract

The purpose of this experiment was to study the effects of dietary protein level on gastrointestinal digestive enzyme activity and jejunal morphology in minks during winter hair period. Sixty healthy male minks at 90 days of age were randomly divided into 6 groups with 10 replicates in each group, and 1 mink in each replicate. The minks in the 6 groups were fed experimental diets containing different levels of protein, which were 28% (groupⅠ), 30% (group Ⅱ), 32% (group Ⅲ), 34% (group Ⅳ), 36% (group Ⅴ) and 38% (group Ⅵ), respectively. There was a 7-day pretrial period followed by a 115-day experimental period. The minks were slaughtered at the end of the feeding experiment. The supernatant of gastrointestinal content was prepared to determine the digestive enzyme activity, and the jejunal tissue section was prepared to observe the jejunal morphology. The results showed as follows: the pepsin activity in the group Ⅴ was significantly higher than that in the groups Ⅰ, Ⅱ and Ⅲ (P<0.01), but no significant difference was found compared with the groups Ⅳ and Ⅵ (P>0.05). The amylopsin activity of duodenum and jejunum in the group Ⅳ was significantly higher than that in the groups Ⅰ, Ⅱ, Ⅲ, Ⅴ and Ⅵ (P<0.01). The amylopsin activity of ileum in the groups Ⅳ and Ⅴ was significantly higher than that in the groups Ⅰ and Ⅱ (P<0.05 or P<0.01). The trypsinase activity of duodenum in the group Ⅳ was significantly higher than that in the groups Ⅰ, Ⅱ, Ⅴ and Ⅵ (P<0.01), and that of jejunum in the group Ⅳ was significantly higher than that in the groups Ⅰ, Ⅱ, Ⅲ, Ⅴ and Ⅵ (P<0.01). The trypsinase activity of ileum in the group Ⅲ was significantly higher than that in the group Ⅵ (P<0.05), but no significant difference was found between group Ⅲ and other groups (P>0.05). The pancrelipase activity of duodenum in the group Ⅵ was significantly higher than that in the groups Ⅱ, Ⅳ and Ⅴ (P<0.05 or P<0.01), and that of jejunum in the group Ⅵ was significantly higher than that in the groups Ⅰ, Ⅱ, Ⅲ, Ⅳ and Ⅴ (P<0.05 or P<0.01). The pancrelipase activity of ileum in the groups Ⅰ, Ⅲ and Ⅴ was significantly higher than that in the groups Ⅱ and Ⅳ (P<0.01), but no significant difference was found compared with the group Ⅳ (P>0.05). The villus height, crypt depth, villus height to crypt depth ratio, mucosal thickness and intestinal wall thickness of jejunum all kept a higher level when dietary protein level was 36%. In conclusion, the dietary protein level can affect the digestive enzyme activity of gastrointestinal tract, and regulate the morphology of jejunum in minks during winter hair period.

Key words： protein level; mink; digestive enzyme; jejunum; morphology

Cite this article

ZHANG Tietao, CUI Hu, YUE Zhigang, YANG Ying, GAO Xiuhua, YANG Fuhe, XING Xiumei . Effects of Dietary Protein Level on Gastrointestinal Digestive Enzyme Activity and Jejunal Morphology in Minks during Winter Hair Period[J]. Chinese Journal of Animal Nutrition, 2012 , 24(2) : 376 -382 . DOI: 10.3969/j.issn.1006-267x.2012.02.027

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