Effects of Chito-Oligosaccharides on Growth Performance, Serum Biochemical Indices and Non-Specific Immunity Function of Rainbow Trout (Oncorhynchus mykiss)

  • LIU Hanliang ,
  • SUN Minmin ,
  • WANG Hongwei ,
  • WAN Wenju ,
  • WANG Jiting
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  • 1. College of Animal Science and Technology, Shandong Agricultural University, Tai'an 271000, China;
    2. Taishan Medical College, Tai'an 271000, China

Received date: 2011-09-16

  Online published: 2012-02-28

Supported by

山东省中青年科学家科研奖励基金(2006BS06018)

Abstract

This experiment was conducted to evaluate the effects of chito-oligosaccharides on growth performance, serum biochemical indices and non-specific immunity function of rainbow trout (Oncorhynchus mykiss). A total of 480 fish with the body weight about 60 g were randomly allotted to 4 groups with 4 replicates each and each replicate contained 30 fish. The fish in the 4 groups were fed a basal diet supplemented with 0, 100, 200 or 400 mg/kg chito-oligosaccharides, respectively. The experiment lasted for 49 days. The results showed as follows: 1) compared with the control group, the specific growth rate in the 100, 200 and 400 mg/kg chito-oligosaccharides groups was increased by 5.60% (P<0.05), 15.89% (P<0.05) and 18.69% (P<0.05), the weight gain rate was increased by 7.53% (P<0.05), 21.21% (P<0.05) and 26.02% (P<0.05), and the protein efficiency rate was increased by 0.60% (P>0.05), 7.02% (P>0.05) and 9.79% (P<0.05), while the feed conversion rate was decreased by 0.60% (P>0.05), 6.55% (P>0.05) and 8.93% (P<0.05), respectively. 2) Dietary supplementation of chito-oligosaccharides improved the contents of total protein, albumin, globulin in serum (P>0.05), while decreased the contents of triglyceride, cholesterol and glucose, and activities of glutamic pyruvic transaminase and glutamic oxaloacetic transaminase in serum (P>0.05). 3) Dietary supplementation of 200 and 400 mg/kg chito-oligosaccharides significantly improved the phagocytic percentage (P<0.05) and phagocytic index of leucocyte (P<0.05), serum bactericidal percentage (P<0.01) and lysozyme activity in serum, liver and gill (P<0.05). 4) No significant differences were found in the contents of moisture, crude protein, crude lipid and ash of whole body among all groups (P>0.05). It is concluded that dietary supplementation of chito-oligosaccharides can improve the growth performance and non-specific immunity function of rainbow trout, and the supplemental level of chito-oligosaccharides in diets is suggested to be 200 mg/kg.

Cite this article

LIU Hanliang , SUN Minmin , WANG Hongwei , WAN Wenju , WANG Jiting . Effects of Chito-Oligosaccharides on Growth Performance, Serum Biochemical Indices and Non-Specific Immunity Function of Rainbow Trout (Oncorhynchus mykiss)[J]. Chinese Journal of Animal Nutrition, 2012 , 24(3) : 479 -486 . DOI: 10.3969/j.issn.1006-267x.2012.03.014

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