Different Preparations of Impatiens balsamina Affect Rumen Microbial Methane Production and Fermentation Characteristics in Vitro

ZHANG Zhihong; HUANG Jiangli; TIAN Xiaojuan; HUANG Huang; ZHANG Guohua; WANG Dongsheng; YIN Yulong; DING Jiannan

doi:10.3969/j.issn.1006-267x.2012.03.023

2012 , Vol. 24 >Issue 3: 550 - 556

DOI: https://doi.org/10.3969/j.issn.1006-267x.2012.03.023

Different Preparations of Impatiens balsamina Affect Rumen Microbial Methane Production and Fermentation Characteristics in Vitro

ZHANG Zhihong ,
HUANG Jiangli ,
TIAN Xiaojuan ,
HUANG Huang ,
ZHANG Guohua ,
WANG Dongsheng ,
YIN Yulong ,
DING Jiannan

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1. Institute of Biological Resource of Jiangxi Academy of Sciences, Nanchang 330029, China;
2. Institute of Subtropical Agriculture, The Chinese Academy of Science, Changsha 410125, China

Received date: 2011-09-21

Online published: 2012-02-28

Supported by

江西省科技支撑计划项目(2010BNA09100);江西省科学院博士基金项目(2010yyb01)

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Abstract

This study was investigated to evaluate the effects of different preparations of Impatiens balsamina on rumen microbial methane (CH₄) production and fermentation characteristics such as pH, ammonia nitrogen (NH₃-N) and microbial crude protein (MCP) in vitro. Rumen fluid was collected from cows fitted with permanent rumen fistulas, and the substrates consisted of straw, corn and soybean as artificial feeds in each fermentation vessel. The medium was supplemented with water, ethanol extract and original plant solid powder at three levels (0.5%, 1.0% and 2.5%), respectively. The results showed that total gas production in the original plant powder preparation was the highest, and 56.8% higher on average than that in the control (CK), and total gas production in ethanol and water extract preparations was respectively 24.7% and 14.1% higher on average than that in CK. Impatiens balsamina preparations obviously increased CO₂, but reduced CH₄ and pH. The trend was more and more obvious with the added amount increasing. The dosage of 2.5% original plant solid powder (S3) completely inhibited CH₄ production. Compared with CK, Impatiens balsamina preparations improved rumen MCP and reduced NH₃-N with the most obvious effect in original plant powder preparation and the worst in water extract preparation. The MCP in original plant solid powder preparation was significantly 84.9%, 139.7% and 199.7% higher than that in CK (P<0.05), but NH₃-N in original plant solid powder preparation was depressed by 41.1%, 52.2% and 55.4% compared with CK (P<0.05), respectively. In conclusion, Impatiens balsamina can reduce CH₄, increase CO₂, promote the conversion of NH₃-N to MCP in rumen microbial fermentation in vitro, and the most effective preparation is original plant powder.

Key words： Impatiens balsamina; rumen fermentation in vitro; methane; microbial crude protein

Cite this article

ZHANG Zhihong , HUANG Jiangli , TIAN Xiaojuan , HUANG Huang , ZHANG Guohua , WANG Dongsheng , YIN Yulong , DING Jiannan . Different Preparations of Impatiens balsamina Affect Rumen Microbial Methane Production and Fermentation Characteristics in Vitro[J]. Chinese Journal of Animal Nutrition, 2012 , 24(3) : 550 -556 . DOI: 10.3969/j.issn.1006-267x.2012.03.023

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