Partial Substitution of Soybean Meal with Distiller's Grains: Influence on Rumen Fermentation Characteristics and Methanogenesis of Buffalo in Vitro

HUANG Yali; ZOU Caixia; HUANG Lianying; LIANG Xianwei; XIA Zhongsheng; WEI Shengju; LIANG Xin

doi:10.3969/j.issn.1006-267x.2012.03.025

2012 , Vol. 24 >Issue 3: 563 - 570

DOI: https://doi.org/10.3969/j.issn.1006-267x.2012.03.025

Partial Substitution of Soybean Meal with Distiller's Grains: Influence on Rumen Fermentation Characteristics and Methanogenesis of Buffalo in Vitro

HUANG Yali ,
ZOU Caixia ,
HUANG Lianying ,
LIANG Xianwei ,
XIA Zhongsheng ,
WEI Shengju ,
LIANG Xin

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1. College of Animal Science and Technology, Guangxi University, Nanning 530005, China;
2. Buffalo Research Institute, Chinese Academy of Agricultural Sciences, Nanning 530001, China

Received date: 2011-09-15

Online published: 2012-02-28

Supported by

农业部948项目(2011-G26);广西科学研究与技术开发项目(桂科攻1123005-2、桂科合1010019-24)

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Abstract

The experiment was conducted to investigate the effects of substitution of soybean meal with distiller's grains at different ratios on rumen fermentation characteristics and methanogenesis of buffalo in vitro. A reading pressure system was used in the in vitro rumen fermentation, and soybean meal in the fermentation substrate was substituted with distiller's grains at ratios of 0, 25%, 50%, 75% and 100% (air-dry basis), respectively. Variation of gas production in 72 h, digestible organic matter content, metabolisable energy, concentrations of ammonia nitrogen, microbial protein and total volatile fatty acid after 24 h cultivation, as well as variation of methane production in 24 h were analyzed. The results showed as follows: 1) accumulation of gas production in 72 h, digestible organic matter content and metabolisable energy reduced with the increasing of substitution ratio of distiller's grains; accumulation of gas production in 72 h of groups 0, 25% and 50% was significantly higher than that of groups 75% (P<0.05) and 100% (P<0.01). 2) Concentration of ammonia nitrogen of group 25% was the highest and significantly higher than that of groups 50% and 75% (P<0.01); concentration of microbial protein of group 0 was the highest and significantly higher than that of groups 75% (P<0.01) and 100% (P<0.05). 3) Concentration of total volatile fatty acids of group 25% was the highest and was significantly higher than that of groups 75% and 100% (P<0.05). 4) Methane production of groups 0%, 25% and 50% at 6 h was significantly higher than that of group 75% (P<0.05); the methane production of groups 75% and 100% at 12 h was significantly higher than that of the other three groups; the methane production of groups 75% and 100% at 24 h was significantly higher than that of groups 50% (P<0.05), 0% (P<0.01) and 25% (P<0.01). In conclusion, distiller's grains can be used as a protein feed for buffalo, and substitution of soybean meal with distiller's grains at ratios of 0 to 50% has no negative effect on rumen fermentation characteristic and methanogenesis.

Key words： distiller’s grains; soybean meal; rumen fermentation; methane

Cite this article

HUANG Yali , ZOU Caixia , HUANG Lianying , LIANG Xianwei , XIA Zhongsheng , WEI Shengju , LIANG Xin . Partial Substitution of Soybean Meal with Distiller's Grains: Influence on Rumen Fermentation Characteristics and Methanogenesis of Buffalo in Vitro[J]. Chinese Journal of Animal Nutrition, 2012 , 24(3) : 563 -570 . DOI: 10.3969/j.issn.1006-267x.2012.03.025

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