Dietary Protein Sources Affect Intestinal Genome-Wide Transcription Profile of Weaner Piglets

SUN Yunzi; YU Bing; CHEN Daiwen; CHEN Xijian

doi:10.3969/j.issn.1006-267x.2012.04.014

Chinese Journal of Animal Nutrition >

2012 , Vol. 24 >Issue 4: 689 - 703

DOI: https://doi.org/10.3969/j.issn.1006-267x.2012.04.014

Dietary Protein Sources Affect Intestinal Genome-Wide Transcription Profile of Weaner Piglets

SUN Yunzi ,
YU Bing ,
CHEN Daiwen ,
CHEN Xijian

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1. Animal Nutrition Institute, Sichuan Agricultural University, Ya’an 625014, China;
2. College of Life Science, Guizhou Normal University, Guiyang 550001, China;
3. Genminix Informatics Ltd. Co. (Shanghai), Shanghai 200234, China

Received date: 2011-10-24

Online published: 2012-03-30

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Abstract

To explore molecular mechanism of dietary protein sources affecting development of intestinal tract of weaner piglets, porcine genome array (PGA) was used to detect intestinal transcription profile of weaner piglets (0, 3, 7 and 14 d after weaning) fed diets with different protein sources. Twenty-four male piglets were randomly divided into two treatments. Piglets in treatment 1 were fed the diet, 30% total protein of which were substituted by milk protein, and those in treatment 2 were fed a diet with whole phytoprotein sources. The results showed as follows: 1) there were 370 differentially expressed genes in treatment 1 (P<0.05). Series test clustering (STC) analysis revealed 26 expression profiles, in which there were 3 significant expression profiles, and significance levels of profiles 5 and 11 (P<0.01) were higher than those of the other profiles. Meanwhile, there were 263 differentially expressed genes in treatment 2 (P<0.05). Twenty-six expression profiles were obtained by STC analysis, in which significance levels of profiles 3 and 22 (P<0.01) were higher than those of the other profiles. 2) Gene co-expression network analysis revealed the molecular regulation mechanism of dietary protein sources affecting intestinal tract development of weaner piglets. A total of 12 and 9 valuable important candidate genes in regulatory network were successfully screened from the piglets in 2 treatments by PGA analysis, and 9 co-expressed genes were also obtained, and their regulation in network were affected by dietary treatment. In conclusion, some potential genes associated with intestinal tract development and function affected by dietary protein sources in weaner piglets are screened, and these candidate genes may deserve further investigation.

Key words： piglet; microarray; gene regulatory network; intestine; development

Cite this article

SUN Yunzi , YU Bing , CHEN Daiwen , CHEN Xijian . Dietary Protein Sources Affect Intestinal Genome-Wide Transcription Profile of Weaner Piglets[J]. Chinese Journal of Animal Nutrition, 2012 , 24(4) : 689 -703 . DOI: 10.3969/j.issn.1006-267x.2012.04.014

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