Retinoic Acid Receptor α and Retinoic Acid Receptor γ in Lateolabrax japonicas: cDNA Cloning and Gene Expression Analysis

  • QIAN Yunxia ,
  • HAN Liu ,
  • TONG Caihuan ,
  • ZHENG Weixian
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  • Key Laboratory of Applied Marine Biotechnology of Ministry of Education, Faculty of Life Science and Biotechnology, Ningbo University, Ningbo 315211, China

Received date: 2011-11-16

  Online published: 2012-03-30

Abstract

This study was conducted to investigate structures of retinoic acid receptor α (RARα) and retinoic acid receptor γ (RARγ) and gene expression profiles in tissues. Reverse transcription PCR (RT-PCR) and rapid amplification of cDNA ends (RACE) methods were applied for the cloning of full-length cDNA coding for RARα and RARγ. Gene expressions of RARα and RARγ in various tissues, such as liver, muscles, heart, eyes, intestine, kidney, fat, spleen, gill and brain, were determined. The results showed as follows: 1) full-length cDNA coding for RARα was 2 094 bp, which included a 124 bp of 5'- untranslated region (UTR), a 608 bp 3'-UTR and a 1 362 bp open reading frame (ORF), the predicted RARα consisted of 453 amino acids with a molecular weight of 56.20 ku, and the theoretical isoelectric point was 8.20. 2) Full-length cDNA coding for RARγ was 1 671 bp consisting of a 36 bp 5'-UTR, a 129 bp 3'-UTR and a 1 506 bp ORF, 501 amino acids with a theoretical isoelectric point of 4.96 and a molecular weight of 56.20 ku was coded. 3) Both RARα and RARγ had typical nuclear receptor structure, and they shared 63.1% identity in their amino acid sequences. Lateolabrax japonicus RARα and RARγ shared high identity (96.9% and 95.2%, respectively) with those of Takifugu rubripes. 4) Lateolabrax japonicus RARα and RARγ gene expressions were detected in all the tested tissues, the higher gene expressions of RARα were in eyes, gill and intestine, the lower values were in heart and brain, and the higher gene expressions of RARγ were in gill and spleen. In conclusion, full-length cDNA of RARα and RARγ in Lateolabrax japonicas are successfully cloned in this study, typical nuclear receptor structure is contained both in RARα and RARγ, and they show ubiquitous tissue distribution characteristics.

Cite this article

QIAN Yunxia , HAN Liu , TONG Caihuan , ZHENG Weixian . Retinoic Acid Receptor α and Retinoic Acid Receptor γ in Lateolabrax japonicas: cDNA Cloning and Gene Expression Analysis[J]. Chinese Journal of Animal Nutrition, 2012 , 24(4) : 712 -721 . DOI: 10.3969/j.issn.1006-267x.2012.04.016

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