Effects of Iron Overload or Deficiency on Serum Biochemical Indices and Liver Hepcidin mRNA Expression of Newborn Piglets

LIU Qinghua; YANG Jianping; LIU Yanhe; ZHU Kuanyou; LI Mengyun

doi:10.3969/j.issn.1006-267x.2012.05.008

2012 >Issue 5: 845 - 851

DOI: https://doi.org/10.3969/j.issn.1006-267x.2012.05.008

Molecular Nutrition

Effects of Iron Overload or Deficiency on Serum Biochemical Indices and Liver Hepcidin mRNA Expression of Newborn Piglets

LIU Qinghua ,
YANG Jianping ,
LIU Yanhe ,
ZHU Kuanyou ,
LI Mengyun

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1. Zhengzhou College of Animal Husbandry and Engineering, Zhengzhou 450011, China;
2. Henan Pollution-Free Feed Engineering Technology Research Center, Zhengzhou 450011, China

Received date: 2011-11-12

Online published: 2012-04-29

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Abstract

This experiment was conducted to investigate the effects of iron overload or deficiency on serum biochemical indices and liver hepcidin mRNA expression of newborn piglets. A total of 15 cross-bred (Duroc×Landrace×Large white) neonatal piglets with an average body weight of (1.22±0.13) kg were randomly divided into 3 groups (iron-deficiency group, regular group and iron-overload group) with 5 replicates per group and 1 piglet per replicate. At 3 and 7 days of age, the piglets in the 3 groups were injected 1 mL physiological saline, 1 mL dextriferron (450 mg iron) and 3 mL dextriferron (450 mg iron), respectively. At 7 days of age, all piglets were killed, and blood, liver and spleen were collected to measure iron content, serum biochemical indices and liver hepcidin mRNA expression. The results showed as follows: iron contents in liver, spleen and serum were all significantly increased with injected Fe content increasing (P<0.05 or P<0.01). Compared with the regular group, the contents of hemoglobin, globulin, total protein and malondialdehyde and the activities of glutathione peroxidase and peroxidase in serum were significantly increased and the superoxide dismutase activity in serum was significantly decreased in iron-overload group (P<0.05 or P<0.01), while the contents of hemoglobin, globulin, total protein and malondialdehyde and the activities of glutathione peroxidase, catalase and peroxidase in serum were significantly decreased and the superoxide dismutase activity in serum was significantly increased in iron-deficiency group (P<0.05 or P<0.01). Compared with the regular group, liver hepcidin mRNA expression was significantly increased in iron-overload group (P<0.01) and significantly decreased in iron-deficiency group (P<0.01). In conclusion, iron overload or deficiency can affect the immune function and anti-oxidant function of newborn piglets; iron overload and deficiency can increase and decrease body’s iron content and liver hepcidin mRNA expression of newborn pigs, respectively.

Key words： newborn piglets; iron overload; iron deficiency; serum biochemical indices; hepcidin mRNA expression

Cite this article

LIU Qinghua , YANG Jianping , LIU Yanhe , ZHU Kuanyou , LI Mengyun . Effects of Iron Overload or Deficiency on Serum Biochemical Indices and Liver Hepcidin mRNA Expression of Newborn Piglets[J]. Chinese Journal of Animal Nutrition, 2012 , (5) : 845 -851 . DOI: 10.3969/j.issn.1006-267x.2012.05.008

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