Arginine Levels Affect Growth and CSN3 Gene Expression of Dairy Cows Mammary Epithelial Cells in Vitro

XU Bolin; WANG Mengzhi; ZHANG Xingfu; HA Si; WANG Chunyan; AO Changjin; WANG Hongrong

doi:10.3969/j.issn.1006-267x.2012.05.009

Chinese Journal of Animal Nutrition >

2012 >Issue 5: 852 - 858

DOI: https://doi.org/10.3969/j.issn.1006-267x.2012.05.009

Molecular Nutrition

Arginine Levels Affect Growth and CSN3 Gene Expression of Dairy Cows Mammary Epithelial Cells in Vitro

XU Bolin ,
WANG Mengzhi ,
ZHANG Xingfu ,
HA Si ,
WANG Chunyan ,
AO Changjin ,
WANG Hongrong

Expand

1. College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, China;
2. State Key Laboratory of Animal Nutrition, Institute of Animal Science, Chinese Academy of Agriculture Science, Beijing 100193, China;
3. College of Animal Science, Inner Mongolia Agricultural University, Inner Mongolia 010018, China

Received date: 2011-12-12

Online published: 2012-04-29

Fold

Abstract

This experiment was conducted to investigate effects of supplementation of arginine (Arg) in culture medium at different levels on proliferation and κ-casein (CSN3) gene expression of mammary epithelial cells (MECs) in vitro. MECs of Chinese Holstein dairy cow were used in this in vitro study. Supplementation levels of Arg were 0.00 (0 group, control group), 69.50 (0.25 group), 139.00 (0.50 group), 278.00 (1.00 group), 556.00 (2.00 group), 1 112.00 (4.00 group) and 2 224.00 mg/L (8.00 group), respectively. The results showed as follows: proliferation of MECs was promoted by Arg, after cultured for 24 h, compared with 0 group, promotion efficiencies of groups 0.25 to 4.00 were significantly higher (P<0.05), while that of 8.00 group was not significantly changed (P>0.05); after cultured for 48 and 72 h, there were significant differences in promotion efficiency between all experimental groups and the control group (P<0.05). Expression level of CSN3 gene was increased by Arg, CSN3 gene expression level of all experimental groups was significantly higher than that of 0 group (P<0.05), and the expression level was highest when the Arg concentration was 556.00 mg/L in culture medium. In conclusion, Arg has a stimulatory effect on proliferation and CSN3 gene expression of MECs. The stimulatory effect on cell proliferation is better when Arg concentration is from 69.50 to 1 112.00 mg/L in culture medium, and the stimulatory effect on CSN3 gene expression is the best when Arg concentration is 556.00 mg/L in culture medium.

Key words： mammary epithelial cell; arginine; cell proliferation; CSN3 gene; gene expression

Cite this article

XU Bolin , WANG Mengzhi , ZHANG Xingfu , HA Si , WANG Chunyan , AO Changjin , WANG Hongrong . Arginine Levels Affect Growth and CSN3 Gene Expression of Dairy Cows Mammary Epithelial Cells in Vitro[J]. Chinese Journal of Animal Nutrition, 2012 , (5) : 852 -858 . DOI: 10.3969/j.issn.1006-267x.2012.05.009

References

[1] WU G,OTT T L,KNABE D A.Amino acid composition of the fetal pig[J].The Journal of Nutrition,1999,129(5):1031-1038.
[2] WU G,POND W G,FLYNN S P.Maternal dietary protein deficiency decreases nitric oxide synthase and ornithine decarboxy-lase activities in placenta and endometrium of pigs during early gestation[J].The Journal of Nutrition,1998,128(12):2395-2402.
[3] MATEO R D,WU G,BAZER F W.Dietary L-arginine supplementation enhances the reproductive performance of gilts[J].The Journal of Nutrition,2007,137(3):652-656.
[4] O'QUINN P R,KNABE D A,WU G.Arginine catabolism in lactating porcine mammary tissue[J].Journal of Animal Science,2002,80(2):467-474.
[5] WU G,KNABE D A,KIM S W.Arginine nutrition in neonatal pigs[J].The Journal of Nutrition,2004,134(Suppl.10):2783S-2790S.

[6] ROOMI M W,IVANOV V,FKALINOVSKY T.Inhibition of matrix metalloproteinase-2 secretion and invasion by human ovarian cancer cell line SK-OV-3 with lysine proline arginine ascorbic acid and green tea extract[J].Journal of Obstetrics and Gynaecology Research,2006,32(2):148-154.

[7] YU L B,DONG X S,SUN W Z.Effect of a nitric oxide synthase inhibitor NG-nitro-L-arginine methyl ester on invasion of human colorectal cancer cell line SL-174T[J].World Journal of Gastroenterology,2005,11(40):6385-6388.
[8] IGNARRO L J,CIRINO G,CASINI A.Nitric oxide as a signaling molecule in the vascular system:an overview[J]. Journal of Cardiovascular Pharmacology,1999,34:879-886.

[9] WU G,MEININGER C J.Arginine nutrition and cardiovascular function[J].The Journal of Nutrition,2000,130(11):2626-2629.
[10] POPOVIC P J,ZEH Ⅲ H J,OCHOA J B.Arginine and immunity[J].The Journal of Nutrition,2007,137(6):1681S-1686S.

[11] BEQUETTE B J,KYLE C E,MACRAE J C,et al.Vascular sources of phenylalanine,tyrosine,lysine,and methinoine for casein synthesis in lactating goats[J].Journal of Dairy Science,1999,82(2):362-377.

[12] MABJEESH S J,KYLE C E,MACRAE J C,et al.Vascular sources of amino acid for milk protein synthesis in goats at two stages of lactation[J].Journal of Dairy Science,2002,85(4):919-929.

[13] 但堂胜.各种动物的理想蛋白及供给[J].国外畜牧科技,1997,24(2):2-6.
[14] ZHOU Y,AKERS R M,JIANG H.Growth hormone can induce expression of four major milk protein genes in transfected MAC-T cells[J].Journal of Dairy Science,2008,91:100-108.

[15] LIV A K K J,SCHMITTGEN T D.Analysis of relative gene expression data using real-time quantitative PCR and the 2^-ΔΔCt method[J].Methods,2001,25(4):402-408.

[16] EHMANN V L,PETERSON W D.To grow mouse mammary epithelial cell in culture[J].Journal of Cell Biology,1984,98(3):1026-1032.

[17] 董矜,田亚平,房征宇.L-Arg对Hela细胞增殖的调控作用研究[J].军医进修学院学报,2004,25(2):100-102.
[18] 何晋,谢秀梅,方叶青,等.L-Arg抑制非对称性二甲基Arg对内皮祖细胞的增殖作用[J].中国动脉硬化杂志,2007,15(10):751-754.
[19] 张剑,何生,李茂德.L-Arg在人肝癌细胞生长增殖中的作用[J].贵阳医学院学报,2003,28(1):5-8.
[20] 罗海吉,周爱军,吉鸿雁.高温下精氨酸对一氧化氮和细胞增殖的影响[J].中国公共卫生,2005,21(3):304-305.
[21] CLARKE S D,ABRAHAM S.Gene expression:nutrient control of pre-and post-transcriptional events[J].FASEB Journal,1992,6:3146-3152.

[22] 来金良.必需氨基酸浓度对奶牛乳腺酪蛋白基因表达的影响.硕士学位论文.杭州:浙江大学,2006.

[23] 杨金勇.蛋氨酸、赖氨酸及其二肤对奶牛乳腺上皮细胞酪蛋白αsl基因表达的影响.硕士学位论文.杭州:浙江大学,2007.

[24] 李喜艳.奶牛乳腺上皮细胞中赖氨酸蛋氨酸配比模式对酪蛋白合成的影响及机理研究.硕士学位论文.北京:中国农业科学院,2011.

[25] MAO J,MOLENAAR A J,WHEELER T T,et al.STAT5 binding contributes to lactational stimulation of promoter Ⅲ expressing the bovine acetyl-CoA carboxylase alpha-encoding gene in the mammary gland[J].Journal of Molecular Endocrinology,2002,29(1):73-88.

[26] BROCKMAN J L,SCHROEDER M D,SCHULER L A.PRL activates the cyclinD1 promoter via the Jak2/Stat pathway[J].Molecular Endocrinology,2002,16(14):774-784.
[27] FRIEDRICHSEN B N,RICHTER H E,HANSEN J A,et al.Signal transducer and activator of transcription 5 activation is sufficient to drive transcriptional induction of cyclin D1 gene and proliferation of rat pancreatic beta-cells[J].Molecular Endocrinology,2003,17(5):945-958.

[28] BUSER A C,GASS-HANDEL E K,WYSZOMIERSKI S L,et al.Progesterone receptor repression of prolactin/signal transducer and activator of transcription 5-mediated transcription of the beta-casein gene in mammary epithelial cells[J].Molecular Endocrinology,2007,21(1):106-125.

Options

Outlines

模态框（Modal）标题

Abstract

Cite this article

References