Effects of Soy Isoflavones on Concentrations of Interferon γ, Interleukin 2 and Interleukin 4 and mRNA Expression of Estrogen Receptor β in Lymphocytes of Spleen and Intestinal Lymph Nodes of Dairy Cows

WANG Ming; SUN Zhigang; LIU Wenqi; YANG Liu; FANG Luoyun; JIANG Linshu

doi:10.3969/j.issn.1006-267x.2012.05.010

2012 >Issue 5: 859 - 869

DOI: https://doi.org/10.3969/j.issn.1006-267x.2012.05.010

Molecular Nutrition

Effects of Soy Isoflavones on Concentrations of Interferon γ, Interleukin 2 and Interleukin 4 and mRNA Expression of Estrogen Receptor β in Lymphocytes of Spleen and Intestinal Lymph Nodes of Dairy Cows

WANG Ming ,
SUN Zhigang ,
LIU Wenqi ,
YANG Liu ,
FANG Luoyun ,
JIANG Linshu

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1. College of Animal Sciences, Laboratory of Molecular Animal Nutrition, Beijing University of Agriculture, Beijing 102206, China;
2. Beijing Capital Agribusiness Group Co., Ltd., Beijing 100029, China

Received date: 2011-12-08

Online published: 2012-04-29

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Abstract

This paper aims to investigate the effects of soy isoflavones on proliferation, activation and production of cytokines in lymphocytes of spleen and intestinal lymph nodes of dairy cows in vitro. A single factor design was used, and lymphocytes were incubated with different levels of soy isoflavones at 37 ℃ in 5% CO₂ for 4, 24 and 48 h, respectively. Concentrations of interferon γ, interleukin 2 and interleukin 4 in culture supernatants were determined by double-antibody sandwich enzyme-linked immunosorbent assay, and expression level of estrogen receptors β mRNA in lymphocytes of spleen and intestinal lymph nodes was determined by real-time PCR. The results showed as follows: 1) after incubated with soy isoflavones (0.25 and 1.00 μg/mL) for a while, concentrations of interferon γ and interleukin 2 in culture supernatants of lymphocytes of spleen were increased at different levels (P<0.05 or P<0.01), and 48 h later, 5.00 and 25.00 μg/mL soy isoflavones reduced interferon γ and interleukin 2 concentrations significantly (P<0.05). 2) In lymphocytes of intestinal lymph nodes, after incubated with soy isoflavones (1.00 and 5.00 μg/mL) for a while, concentrations of interferon γ and interleukin 2 were increased at different levels (P<0.05 or P<0.01); the concentrations tended to be increased by 0.25 μg/mL soy isoflavones (P>0.05); 48 h later, concentrations of interferon γ (P<0.05) and interleukin 2 (P<0.01) were significantly decreased. 3) Compared with that in the control group, interleukin 4 concentration in lymphocytes of spleen in experimental groups were not change significantly (P>0.05), and the concentration in lymph nodes of intestinal lymph nodes in experimental groups was significantly reduced (P<0.05 or P<0.01). 4) Ratios of interferon γ and interleukin 4 in lymphocytes of spleen tended to be increased, but that of intestinal lymph nodes tended to be opposite. 5) Expression levels of estrogen receptors β mRNA in lymphocytes of spleen and intestinal lymph nodes were significantly increased (P<0.05 or P<0.01), and there was positive correlation between levels of estrogen receptors β mRNA expression and soy isoflavones. In conclusion, soy isoflavones can increase concentrations of interferon γ and interleukin 2, ratio of interferon γ and interleukin 2, as well as expression level of estrogen receptors β mRNA, but can decrease interleukin 4 concentration; low dose (0.25 to 5.00 μg/mL) soy isoflavones can get better effects on immunity promotion, but high dose (25.00 to 100.00 μg/mL) can stimulate expression of estrogen receptors β mRNA

Key words： soy isoflavones; interferon γ; interleukin 2; interleukin 4; estrogen receptors β; immunity

Cite this article

WANG Ming , SUN Zhigang , LIU Wenqi , YANG Liu , FANG Luoyun , JIANG Linshu . Effects of Soy Isoflavones on Concentrations of Interferon γ, Interleukin 2 and Interleukin 4 and mRNA Expression of Estrogen Receptor β in Lymphocytes of Spleen and Intestinal Lymph Nodes of Dairy Cows[J]. Chinese Journal of Animal Nutrition, 2012 , (5) : 859 -869 . DOI: 10.3969/j.issn.1006-267x.2012.05.010

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