Different Bacillus Probiotics Affect Growth Performance and Ammonia Emission from Excreta of Broilers

TAN Li; YUAN Dong; ZHANG Yunbing; ZHAN Xiu

doi:10.3969/j.issn.1006-267x.2012.05.012

Chinese Journal of Animal Nutrition >

2012 >Issue 5: 877 - 885

DOI: https://doi.org/10.3969/j.issn.1006-267x.2012.05.012

Feed Science and Technology

Different Bacillus Probiotics Affect Growth Performance and Ammonia Emission from Excreta of Broilers

TAN Li ,
YUAN Dong ,
ZHANG Yunbing ,
ZHAN Xiu

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1. Institute of Feed Science, Zhejiang University, Hangzhou 310058, China;
2. Zhejiang Xinxin Feed Co., Ltd., Jiaxing 314005, China

Received date: 2011-12-08

Online published: 2012-04-29

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Abstract

A comparative study was conducted to investigate the effects of different Bacillus probiotics on growth performance and ammonia emission from excreta of broilers and discussed its possible mechanism. Eight hundred and forty 1-day-old Lingnan yellow-feathered broilers were randomly allotted to 4 groups with 3 replicates. The broilers in the control group were fed a basal diet supplemented with virginiamycin and roxarsone, and those in experimental groups were fed the basal diet supplemented with Clostridium butyricum (Clostridium butyricum group, CBG), Bacillus licheniformis (Bacillus licheniformis group, BLG) or B. subtilis (B. subtilis group, BSG), respectively. The experiment lasted for 50 days, including a starter period (1 to 21 days of age) and a finisher period (22 to 50 days of age). The results showed as follows: 1) compared with the control group, average daily feed intake and average daily gain of broilers in BLG and BSG were significantly increased (P<0.05) and there was an increasing trend in the ratio of feed to gain (P>0.05) during the starter period. There were no significant differences in all the indices about growth performance during the whole and finisher period between experimental groups and the control group (P>0.05), but those in CBG were the best. 2) The number of Coliforms, total aerobe, total anaerobe and pH of caecum in the experimental groups were not affected (P>0.05), but the number of Lactobacillus was increased significantly in CBG at 21 and 50 days of age (P<0.05); pH of caecum and feces in CBG was the lowest among all groups. 3) Anthine oxidase activity and uric acid content in serum in experimental groups were increased whereas the serum urea nitrogen content was decreased to a certain extent, furthermore, uric acid content in caecum and feces in experimental groups were decreased whereas urea nitrogen content in feces was increased to a certain extent; fecal ammonia nitrogen content in BLG and BSG was significantly increased at 50 days of age (P<0.05), but no significant difference was found in that between CBG and the control group (P>0.05). 4) There was no significant difference in ammonia emission from excreta among all groups at 12, 19 and 25 days of age (P>0.05), and the NH₃ loss in BSG was the highest of all groups and that in CBG tended to reduce at 33, 40 and 47 days of age compared with the control group (P>0.05). In conclusion, Bacillus probiotics can regulate the intestinal miroflora, affect the nitrogen metabolism in vivo and decrease pH of caecum and feces, and C. butyricum can reduce the ammonia emission from excreta and come out to be the best probiotic in the present study.

Key words： Bacillus; broilers; growth performance; ammonia emission; mechanism

Cite this article

TAN Li , YUAN Dong , ZHANG Yunbing , ZHAN Xiu . Different Bacillus Probiotics Affect Growth Performance and Ammonia Emission from Excreta of Broilers[J]. Chinese Journal of Animal Nutrition, 2012 , (5) : 877 -885 . DOI: 10.3969/j.issn.1006-267x.2012.05.012

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