This study was to investigate the effects of housefly larvae products on nutrient availability, intestinal flora and serum biochemical indices in yellow-feathered broilers. Two hundred and fifty-two one-day-old yellow-feathered broilers were allotted to 7 groups with 3 replicates in each group and 12 broilers in each replicate. The broilers in control group were fed a basal diet, and those in the other six groups were fed basal diets with 0.2% and 0.5% housefly larvae enzymatic hydrolysis peptides, 0.2% and 0.5% housefly larvae hydrolyzated residues, and 0.2% and 0.5% degreasing housefly larvae powder instead of commensurate fish meal in basal diet, respectively. The results showed as follows: 1) with increased dosage of housefly larvae enzymatic hydrolysis peptides, there was a increasing tendency in dry matter, crude protein and energy availability of broilers in the groups of housefly larvae enzymatic hydrolysis peptides compared with the control group (P>0.05); 0.5% housefly larvae enzymatic hydrolysis peptides significantly increased the availability of ether extract, calcium, phosphorus and ash (P<0.05). 2) The number of Lactobacillus and Bifidobacterium was increased (P<0.05), and that of Escherichia coli and Salmonella in the intestine of broilers fed the diets supplemented with housefly larvae enzymatic hydrolysis peptides was decreased at weeks 2 and 4 of the experiment (P<0.05). 3) Serum alanine aminotransferase activity of broilers fed the diets supplemented with housefly larvae enzymatic hydrolysis peptides was decreased (P<0.05), and serum total protein content was increased at week 2 of the experiment (P<0.05), and the optimal result was obtained in the group of 0.5% housefly larvae enzymatic hydrolysis peptides. Compared with the control group, urine nitrogen content in serum of broilers in experimental groups was decreased (P<0.05). Obviously, housefly larvae enzymatic hydrolysis peptides can improve nutrient availability and intestinal flora in broilers, and better effect is obtained in the group of 0.5% housefly larvae enzymatic hydrolysis peptides.