Dietary Conjugated Linoleic Acid Level Affects Performance, Eggshell Quality and Serum Biochemical Indices of Laying Hens

LIU Xuelan; SHI Tianhong; JING Qingchuan; YAN Peipei; WEI Xiangfa; LIU Ruiting

doi:10.3969/j.issn.1006-267x.2012.05.018

2012 >Issue 5: 926 - 932

DOI: https://doi.org/10.3969/j.issn.1006-267x.2012.05.018

Short Communications

Dietary Conjugated Linoleic Acid Level Affects Performance, Eggshell Quality and Serum Biochemical Indices of Laying Hens

LIU Xuelan ,
SHI Tianhong ,
JING Qingchuan ,
YAN Peipei ,
WEI Xiangfa ,
LIU Ruiting

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Poultry Institute, Shandong Academy of Agricultural Sciences, Jinan 250023, China

Received date: 2011-11-25

Online published: 2012-04-29

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Abstract

This experiment was conducted to investigate the effects of the supplementation of conjugated linoleic acid (CLA) on the performance, eggshell quality, and its mechanism. Four hundred and eighty healthy 40-week-old Hy-Line White laying hens with similar body weight and laying rate were divided randomly into 4 groups with 3 replicates per group and 40 birds in each replicate. The control diet had no CLA, and the experimental diets contained 0.5%, 1.0% and 2.0% CLA, respectively. Eight weeks later, six of each group was slaughtered and the blood was collected to determine the contents of thiothyone (T₃), tetraiodothyronine (T₄), cortisol, estradiol (E₂), Ca²⁺and P⁵⁺, the alkaline phosphatase activity (ALP) in serum, and the carnitine palmitoyltransterase (CPT-Ⅰ) activity in liver. The results showed as follows: 1) there was no significant difference in performance between the control group and experimental groups (P>0.05). 2) Compared with the control group, the eggshell strength in experimental groups was significantly improved (P<0.05), but there was no significant difference in eggshell thickness between the control group and experimental groups (P>0.05). 3) The contents of T₃, T₄, cortisol and E₂ in serum of laying hens in 2.0% CLA group were significant higher than those in the other groups (P<0.05); there were no significant differences in the contents of calcium ions and inorganic phosphorus ions in serum of laying hens between the control group and experimental groups (P>0.05); compared with the control group, the ALP activity in serum of laying hens in experimental groups was significantly increased (P<0.05) except for 2.0% CLA group. 4) The CPT-Ⅰ activity in liver of laying hens was significantly lower than that in the control group (P<0.05). It is concluded that 1.0% CLA can significantly increase serum ALP activity, and improve serum E₂ content, so it can significantly improve the eggshell strength, furthermore, it has no bad effect on performance of layers.

Key words： conjugated linoleic acid; performance; eggshell quality; serum biochemical indices; alkaline phosphates; endocrine hormones; carnation palmitoyltransterase

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LIU Xuelan , SHI Tianhong , JING Qingchuan , YAN Peipei , WEI Xiangfa , LIU Ruiting . Dietary Conjugated Linoleic Acid Level Affects Performance, Eggshell Quality and Serum Biochemical Indices of Laying Hens[J]. Chinese Journal of Animal Nutrition, 2012 , (5) : 926 -932 . DOI: 10.3969/j.issn.1006-267x.2012.05.018

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