High Calcium Diet Affects Serum Nitric Oxide Concentration and Antioxidant Function of Pullets

GUO Xiaoquan; HUANG Kehe; CAO Huabin; HU Guoliang; LI Haotang; ZHANG Caiying

doi:10.3969/j.issn.1006-267x.2012.05.019

Chinese Journal of Animal Nutrition >

2012 >Issue 5: 933 - 938

DOI: https://doi.org/10.3969/j.issn.1006-267x.2012.05.019

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High Calcium Diet Affects Serum Nitric Oxide Concentration and Antioxidant Function of Pullets

GUO Xiaoquan ,
HUANG Kehe ,
CAO Huabin ,
HU Guoliang ,
LI Haotang ,
ZHANG Caiying

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1. College of Animal Science and Technology, Jiangxi Agricultural University, Nanchang 330045, China;
2. Institute of Nutritional and Metabolic Disorders in Animals and Fowls, Nanjing Agricultural University, Nanjing 210095, China

Received date: 2012-02-01

Online published: 2012-04-29

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Abstract

This experiment was conducted to study the effects of high calcium diet on nitric oxide (NO) concentration and antioxidant function of pullets. One hundred healthy pullets aged 35 days were randomly divided into 2 groups with 5 replicates in each group and 10 chickens per replicate. The pullets in different groups were fed normal calcium diet (control group, 1.00% calcium) and high calcium diet (HC group, 3.78% calcium), respectively. Healthy situations of the pullets were inspected daily. NO concentration, malondialdehyde (MDA) content and enzymatic antioxidant activity in serum were detected on days 0, 8, 16, 24 and 32 after feeding high calcium diet. The results showed as follows: 1) high calcium diet could cause diarrhea. Pullets would be hypercalcemia on day 8 after feeding high calcium diet. 2) There was no significant difference in serum NO concentration of pullets between HC group and the control group on days 8 and 16 after feeding high calcium diet (P>0.05), but that in HC group was significantly increased on days 24 and 32 after feeding high calcium diet (P<0.05). There were no significant differences in serum superoxide dismutase (SOD) activity and total anti-oxidation capability (T-AOC) of pullets between HC group and the control group on days 8 and 24 after feeding high calcium diet (P>0.05), but those in HC group were increased on day 16 after feeding high calcium diet (P<0.05), meanwhile, decreased on day 32 after feeding high calcium diet compared with the control group (P<0.05). There was no significant difference in serum MDA content of pullets between HC group and the control group on days 8, 16 and 24 after feeding high calcium diet (P>0.05), but that in HC group was significantly increased on day 32 after feeding high calcium diet (P<0.05). There was a increasing trend in serum xanthine oxidase (XOD) activity in HC group on days 24 and 32 after feeding high calcium diet compared with the control group (P>0.05). The results indicate that high calcium diet can cause long-term diarrhea, increase the lipid peroxidation, and decrease the enzymatic antioxidant activity.

Key words： high calcium diet; pullets; NO concentration; antioxidant function

Cite this article

GUO Xiaoquan , HUANG Kehe , CAO Huabin , HU Guoliang , LI Haotang , ZHANG Caiying . High Calcium Diet Affects Serum Nitric Oxide Concentration and Antioxidant Function of Pullets[J]. Chinese Journal of Animal Nutrition, 2012 , (5) : 933 -938 . DOI: 10.3969/j.issn.1006-267x.2012.05.019

References

[1] WILLIAMS B,WADDINGTON D,SOLOMIN C.Dietary effects on bone quality and turnover,and Ca and P metabolism in chickens[J].Research in Veterinary Science,2000,69:81-87.

[2] WIDEMAN R F,BUSS E G.Aterial blood gas,pH,and bicarbonate values in laying hens selected for thick or thin eggshell production[J].Poultry Science,1985,64:1015-1019.

[3] 张玲,王志跃.饲粮不同粗蛋白质和钙水平对种公鸡繁殖性能的影响[J].动物营养学报,2011,23(8):1280-1288.
[4] GUO X Q,HUANG K H,TANG J X.Clinicopathology of gout in growing layers induced by high calcium and high protein diets[J].British Poultry Science,2005,465:641-646.

[5] WIDEMAN R F,CLOSSER J A,ROUSH W B,et al.Urolithiasis in pullets and laying hens:role of dietary calcium and phosphorus[J].Poultry Science,1985,64:2300-2307.

[6] 郭小权,黄克和,骆建兵,等.高钙高蛋白日粮对青年蛋鸡体液酸碱度和电解质的影响[J].西北农林科技大学学报:自然科学版,2005,33(10):80-84.
[7] 郭小权,黄克和,陈甫,等.高钙日粮致青年蛋肾脏损伤的超微结构病理学变化[J].江西农业大学学报,2008,30(2):311-314.
[8] 方允中,郑荣梁.自由基生物学的理论与应用[M].北京:科学出版社,2002:822-897.

[9] SAKAGUCHI S,TSUTSUMI E,YOKOTA K.Degense effects of a traditional Chinese medicine (sho-saiko-to) against metablic disorders during endotoxemia;approached from the behavior of the calcium ion[J].Biological and Pharmaceutical Bulletin,1994,17(2):232-236.

[10] GUO X,HUANG K,CHEN H,et al.High dietary calcium causes metabolic alkalosis in egg-type pullets[J].Poultry Science,2008,87:1353-1357.

[11] KOSHLAND D E,Jr.The molecular of the year[J].Science,1992,258:1861.

[12] MONCADA S,HIGGS A.The L-arginine-nitric oxide pathway[J].New England Journal of Medcine,1993,329:2002-2012.

[13] HALLIWELL B.Oxygen radicals,nitric oxide and human inflammatory joint disease[J].Annals of the Rheumatic Diseases,1995,54:505-510.

[14] SHIELLS R,FALK G.Retinalon-bipolar cells contain nitric oxide-sensitive guanylate cyclase[J].Neuroreport,1992,3(10):845-848.

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