Effects of Nano-Ceria on Activity and Gene Expression of Pancreatic Lipase in Hens

CHEN Hui; WANG Cuiju; WU Pengwei; DI Keqian; BAO Huiling; HUANG Renlu

doi:10.3969/j.issn.1006-267x.2012.05.020

Chinese Journal of Animal Nutrition >

2012 >Issue 5: 939 - 945

DOI: https://doi.org/10.3969/j.issn.1006-267x.2012.05.020

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Effects of Nano-Ceria on Activity and Gene Expression of Pancreatic Lipase in Hens

CHEN Hui ,
WANG Cuiju ,
WU Pengwei ,
DI Keqian ,
BAO Huiling ,
HUANG Renlu

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1. College of Animal Science and Technology, Agricultural University of Hebei, Baoding 071001, China;
2. Hebei University, Baoding 071001, China;
3. Shijiazhuang Municipal Animal Husbandry and Aquatic Products Bureau, Shijiazhuang 052160, China

Received date: 2011-11-21

Online published: 2012-04-29

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Abstract

A test method of pancreatic lipase by real-time fluorescence quantitative PCR was established to investigate the effects of nano-ceria on activity and gene expression of pancreatic lipase in hens. A total of 384 healthy commercial hens at 26 weeks of age were selected and randomly allotted to 4 groups with 8 replicates each group and 12 hens per replicate. The basal diet was a corn-soybean meal diet. The experimental diets were the basal diet supplemented with 0 (group Ⅰ), 30 (group Ⅱ), 60 (group Ⅲ) and 90 mg/kg (group Ⅳ) nano-ceria, respectively. The experiment lasted for 7 weeks. The results showed as follows: 1) this study had established the test method to examine the mRNA expression level of pancreatic lipase using real-time fluorescence quantitative PCR, and this test method had some advantages such as good linear relationship, specificity, sensitivity and reproducibility; 2) the pancreatic lipase activity in group Ⅲ was significantly higher than that in groups Ⅰ and Ⅳ (P<0.05 or P<0.01), and the pancreatic lipase mRNA expression level in group Ⅲ was significantly higher than that in other groups(P<0.05). The results indicate that supplementation of 60 mg/kg nano-ceria in the diet can significantly improve the pancreatic lipase activity and up-regulate the pancreatic lipase mRNA expression level.

Key words： nano-ceria; pancreatic lipase; activity; gene expression

Cite this article

CHEN Hui , WANG Cuiju , WU Pengwei , DI Keqian , BAO Huiling , HUANG Renlu . Effects of Nano-Ceria on Activity and Gene Expression of Pancreatic Lipase in Hens[J]. Chinese Journal of Animal Nutrition, 2012 , (5) : 939 -945 . DOI: 10.3969/j.issn.1006-267x.2012.05.020

References

[1] 李西风,朱宴辰.稀土元素在动物营养中的作用[J].中国饲料,1998(1):24.

[2] 宋智娟,赵国先,张晓云,等.饲料添加剂稀土的研究进展[J].饲料博览,2005(9):41-43.

[3] 洪法水,王雪峰,吴康,等.重金属离子对猪胰α-淀粉酶活性影响的作用机理研究[J].高等学校化学学报,2001,22(12):1979-1983.
[4] 洪法水,王雪峰,沈颂东,等.Pb²⁺、Cd²⁺和Ce³⁺对猪胰α-淀粉酶活性的影响[J].生物化学与生物物理进展,2002,29(1):74-77.
[5] 王雪峰,洪法水,沈颂东,等.铈对猪胰α-淀粉酶活性影响的研究[J].中国稀土学报,2002,20(4):353-356.
[6] 王建辉,吕武兴.纳米氧化锌在动物营养中的应用前景[J].饲料广角,2003(18):21-23.

[7] FLEIGE S,PFAFFL M W.RNA integrity and the effect on the real-time qRT-PCR performance[J].Molecular Aspects of Medicine,2006,27:126-139.

[8] 江振莹,贾志海,郭云锁,等.稀土元素对鲤鱼肠道发育及消化酶活性的影响[J].动物营养学报,2007,19(1):86-90.
[9] 万辉.稀土壳糖胺螯合盐对蛋鸡生产性能及营养物质利用的影响.硕士学位论文.武汉:华中农业大学,2008.

[10] 刘军,陈爱敬,胡兵,等.稀土壳聚糖螯合盐对鲫鱼消化酶活力的影响[J].中国饲料,2008(6):35-38.

[11] FENDRI A,FRIKHA F,MOSBAH N,et al.Biochemical characterization,cloning,and molecular modellin of chicken pancreatic lipase[J].Archives of Biochemistry and Biophysics,2006,451(2):149-159.

[12] SABB J E,GODFREY P M,BRANNON P M.Adaptive response of rat pancreatic lipase to dietary fat:effect of amount and type of fat[J].The Journal of Nutrition,1986,116(1):892-899.
[13] WICKER C,SCHEELE G A,PUIGSERVER A.Pancreatic adaptation to dietary lipids is mediated by changes in lipase mRNA[J].Biochimie,1988,70(9):1277-1283.

[14] WICKER C,PUIGSERVER A.Expression of rat pancreatic lipase gene is modulated by a lipid-rich diet at a transcriptional level[J].Biochemical and Biophysical Research Communications,1990,166:358-364.

[15] RICKETTS J,BRANNON P M.Amount and type of dietary fat regulate pancreatic lipase gene expression in rat[J].The Journal of Nutrition,1994,124(8):1166-1171.
[16] RIPPE C,BERGER K,MEI J,et al.Effect of long-term high-fat feeding on the expression of pancreatic lipase and adipose tissue uncoupling proteins in mice[J].Pancreas,2003,26(2):e36-e42.

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