Dietary Crude Protein Level Affects Milk Yield and Nitrogen Metabolism of Lactating Water Buffalo

ZOU Caixia; WEI Shengju; LIANG Xianwei; QIN Guangsheng; YANG Bingzhuang; YANG Chengjian

doi:10.3969/j.issn.1006-267x.2012.05.021

Chinese Journal of Animal Nutrition >

2012 >Issue 5: 946 - 952

DOI: https://doi.org/10.3969/j.issn.1006-267x.2012.05.021

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Dietary Crude Protein Level Affects Milk Yield and Nitrogen Metabolism of Lactating Water Buffalo

ZOU Caixia ,
WEI Shengju ,
LIANG Xianwei ,
QIN Guangsheng ,
YANG Bingzhuang ,
YANG Chengjian

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Buffalo Research Institute, Chinese Academy of Agricultural Sciences, Ministry of Agriculture (Guangxi) Key Laboratory of Buffalo Genetic and Breedin, Nanning 530001, China

Received date: 2011-11-11

Online published: 2012-04-29

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Abstract

This experiment was conducted to investigate the effects of dietary crude protein level on milk yield and nitrogen metabolism of lactating water buffalo. A 4×4 Latin square experiment design was used in the experiment. Sixteen healthy early lactation water buffalo with similar milk yield in the last lactation and 2 or 3 parities were divided into 4 groups to carry out animal experiment, and digestion and metabolism experiment. The animals were randomly divided into 4 groups and fed diets containing varying levels of crude protein (16.0%, 15.2%, 14.4% and 13.6%). There were 4 feeding trial periods, each period included 21 d with 7 d adaptation period, and whole experiment lasted for 84 d. According to Latin square experiment design, each group in each period was fed different levels of dietary crude protein. Two nitrogen digestion and metabolism trials were conducted on the last 4 days of the second and the fourth feeding trial period. The results showed that there were significant differences in total nitrogen intake, digestible nitrogen, milk nitrogen/total nitrogen intake and apparent nitrogen digestibility among some groups (P<0.05); there were no significant differences in milk nitrogen, fecal nitrogen, urinary nitrogen among each group(P>0.05). There were no significant differences in milk yield, milk protein percentage, milk fat percentage, milk non-solid percentage whole milk solids content and lactose percentage among each group (P>0.05). There were no significant differences in the contents of serum total protein and urea nitrogen (P>0.05). The relationship between nitrogen intake (x, g/d) and fat corrected milk (y, kg/d) was showed as follows: y=－0.001 6x²+0.955 6x-129.91. In conclusion, dietary crude protein level has no significant effect on performance and blood biochemical indices of lactating water buffalo, according to the curvilinear relationship between nitrogen intake and fat corrected milk, when the nitrogen intake is 298.625 g/d, the max standard milk yield of water buffalo is 12.773 kg/d.

Key words： water buffalo; early lactation; nitrogen metabolism; milk yield

Cite this article

ZOU Caixia , WEI Shengju , LIANG Xianwei , QIN Guangsheng , YANG Bingzhuang , YANG Chengjian . Dietary Crude Protein Level Affects Milk Yield and Nitrogen Metabolism of Lactating Water Buffalo[J]. Chinese Journal of Animal Nutrition, 2012 , (5) : 946 -952 . DOI: 10.3969/j.issn.1006-267x.2012.05.021

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