Comparison of Digestive Enzyme Activities in Normal and Growth Retarded Japanese Eels (Anguilla japonics)

HUANG Yongchun

doi:10.3969/j.issn.1006-267x.2012.05.025

Chinese Journal of Animal Nutrition >

2012 >Issue 5: 977 - 982

DOI: https://doi.org/10.3969/j.issn.1006-267x.2012.05.025

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Comparison of Digestive Enzyme Activities in Normal and Growth Retarded Japanese Eels (Anguilla japonics)

HUANG Yongchun

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Fishery College, Jimei University, Xiamen 361021, China

Received date: 2011-11-11

Online published: 2012-04-29

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Abstract

This experiment was conducted to study the effects of temperatures on the activities of digestive enzymes in Japanese eels Anguilla japonics (n=20) and growth retarded eels (n=20). The weight of the normal eels was from 126.4 to 140.2 g per eel, and that of growth retarded eels was from 3.5 to 8.6 g per eel. The activities of protease, amylase, and lipase in the normal eels and growth retarded eels under the condition of different temperatures (5, 10, 15, 25, 30, 35, 40, 45 and 55 ℃) were studied. The results showed as follows: with the increasing of temperature, the digestive enzyme activities were increased at first, and then decreased. The optimum temperature of the protease in hepatopancreas, intestine and stomach was 45 ℃. In the same experimental temperature, the order of protease activity was intestine>stomach>hepatopancreas except growth retarded eels at 5, 15 and 55 ℃. The protease activity in hepatopancreas, intestine and stomach of growth retarded eels was only 29.5%, 15.7% and 25.2% of that of the normal eels, respectively (P<0.05). The optimum temperature of amylase activity was 30 ℃, and the order of amylase activity of the normal eels was hepatopancreas>intestine>stomach in experimental temperatures except at 5 ℃, but the order of amylase activity of growth retarded eels was intestine>hepatopancreas>stomach in experimental temperatures. The amylase activity in hepatopancreas, intestine and stomach of growth retarded eels was only 42.4%, 73.7% and 43.8% of that of the normal eels, respectively (P<0.05). The optimum temperature of lipase activity was 35 ℃, and the order of lipase activity of the normal eels was hepatopancreas>stomach>intestine in experimental temperatures, but the order of lipase activity of growth retarded eels was hepatopancreas>intestine>stomach in experimental temperatures except at 35 ℃; the lipase activity in hepatopancreas, intestine and stomach of growth retarded eels was only 41.5%, 45.6% and 23.2% of that of the normal eels, respectively (P<0.05). These results indicate that the activities of protease, amylase and lipase in hepatopancreas, intestine and stomach of growth retarded eels are significantly lower than those of normal eels, which directly affect their growth and development.

Key words： temperature; Anguilla japonics; growth retarded eels; digestive enzyme activities

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HUANG Yongchun . Comparison of Digestive Enzyme Activities in Normal and Growth Retarded Japanese Eels (Anguilla japonics)[J]. Chinese Journal of Animal Nutrition, 2012 , (5) : 977 -982 . DOI: 10.3969/j.issn.1006-267x.2012.05.025

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