The effects of vitamin, mineral, and energy and protein levels on reproductive performance, blood reproductive hormone concentrations and related gene mRNA relative expression in reproductive axis of female Zhedong white goose were determined by adding vitamin and mineral, and regulating dietary energy and protein level. One hundred and thirty-eight female Zhedong white geese with the same month old were divided into 3 groups according to their body weight, which were provided 3 kinds of experimental diets. In the experimental period (150 d), the changes of reproductive performance (average egg number, average egg weight, fertility rate and hatchability rate), concentrations of blood reproductive hormones [follicle stimulating hormone (FSH), luteinizing hormone (LH), progesterone (P4), estrogen (E2) and prolactin (PRL)] and mRNA relative expression of related genes [gonadotropin releasing hormone (GnRH), follicle stimulating hormone-β (FSHβ), estrogen receptor 1 (ESR1), estrogen receptor 2 (ESR2), follicle stimulating hormone receptor (FSHR), PRL and prolactin receptor (PRLR) ] in reproductive axis were measured. The results showed as follows: 1) adding vitamin and mineral significantly increased average egg weight and fertility rate during the 1st laying cycle (P<0.05), increased concentrations of blood FSH and P4, decreased concentration of blood LH, changed E2, P4 and PRL pattern during the 2nd laying cycle (P<0.05), down-regulated GnRH, PRL and PRLR mRNA relative expression in the hypothalamus, pituitary and ovary, respectively, and up-regulated ESR2 mRNA relative expression in the ovary (P<0.05). 2) Regulating energy and protein level significantly increased average egg weight during the 2nd laying cycle (P<0.05), increased concentration of blood LH, decreased concentration of blood FSH, changed P4 and E2 pattern during the 2nd laying cycle (P<0.05), increased GnRH, PRL and PRLR mRNA relative expression in the hypothalamus and pituitary, and decreased FSHR mRNA relative expression in the ovary (P<0.05). The results of this study indicate that adding vitamin and mineral and regulating dietary energy and protein level improve reproductive performance by partly affecting concentrations and patterns of blood reproductive hormones, and local regulating related gene mRNA relative expression in reproductive axis of the female Zhedong white geese.
ZHAO Xin
,
SHAO Tao
,
WANG Yaqin
,
LUO Jinbiao
,
CHEN Weihu
,
SUN Hongxia
,
ZHOU Weidong
. Effects of Vitamin, Mineral, and Energy and Protein Levels on Reproductive Performance, Blood Reproductive Hormone Concentrations and Related Gene mRNA Relative Expression in Reproductive Axis of Female Zhedong White Geese[J]. Chinese Journal of Animal Nutrition, 2012
, 24(6)
: 1110
-1118
.
DOI: 10.3969/j.issn.1006-267x.2012.06.017
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