This experiment was conducted to study the effects of dietary ginkgo defensins supplementation on growth performance and immune parameters of weaner piglets, and aimed to provide safety, environmental, efficient green feed additive for feed production. A total of 120 weaner piglets (Duroc×Landrace×Yorkshire) with average weight about 7 kg were randomly divided into a control group and a experimental group, and each group contained 5 replicates and 12 piglets in each replicate. Piglets in the control group were fed a basal diet, and the piglets in the experimental group were fed the basal diet supplemented with 0.2% ginkgo defensins. The experiment lasted for 21 days. The results showed as follows: compared with the control group, the final weight in the experimental group was increased by 8.92% (P<0.05), the diarrhea rate was significantly decreased (P<0.01), and feed to gain ratio was decreased by 7.27% (P<0.05).The contents of serum immunoglobulin G, immunoglobulin M, and immunoglobulin A in the experimental group were increased by 13.03% (P<0.05), 12.27% (P<0.05), and 7.89% (P>0.05), respectively; the contents of serum albumin and total protein were increased by 19.97% (P<0.05), 14.97% (P<0.05), respectively. The blood T-lymphocyte transformation rate in the experimental group was increased by 8.25% (P>0.05), and interleukin 2 content was increased by 15.25% (P<0.01). In conclusion, ginkgo defensins can improve growth performance and immune parameters, and reduce the diarrhea rate.
CAO Manhu
,
WANG Cai
,
XIAO Xiaoping
,
PI Canhui
. Ginkgo Defensins Affect Growth Performance and Immune Parameters of Piglets[J]. Chinese Journal of Animal Nutrition, 2012
, 24(6)
: 1119
-1123
.
DOI: 10.3969/j.issn.1006-267x.2012.06.018
[1] 闻晓波,崔玉东.防御素研究进展[J].国外医学:免疫学分册,2002,25(6):297-300.
[2] 常新霞,董碧蓉.β-防御素:治疗感染的新希望[J].华西医学,2004,19(1):172-173.
[3] DIAMOND G,KAISER V,RHODES J,et al.Transcriptional regution of β-defensin gene expression is trchel epithelial cells[J].Infection and Immunity,2000,68:113-122.
[4] 李思明,周定刚.维生素D3对丝毛乌骨鸡组织中β-防御素基因的表达调控[J].动物营养学报,2009,21(04):573-579.
[5] 安沙,韩菲菲,高彦华,等.β-防御素在金华猪和长白猪肠道中表达规律的研究[J].动物营养学报,2011,23(10):1762-1768.
[6] WEI G,DE LEEUW E,PAZGIER M,et al.Through the lookingglass,mechanistic insights from enantiomeric human defensins[J].Journal of Biological Chemistry,2009,284(42):29180-29192.
[7] BREUKINK E,DE KRUIJFF B.Lipid Ⅱ as a target for antibiotics[J].Nature Reviews Drug Discovery,2006,5(4):321-332.
[8] 余兴邦,郭锁链,乌翠兰,等.防御素研究进展[J].动物医学进展,2006,27(8):47-51.
[9] ASHITANI J,NAKAZATO M,MUKAE H,et al.Recombinant granulocyte colony-stimulating factorinduces production of human neutrophil peptides in lung cancer patients with neutropenia[J].Regulatory Toxicology and Pharmacology,2000,95(1/2/3):87-92.
[10] 龙晶,杜立新.哺乳动物防御素研究进展[J].中国畜牧兽医,2007,34(8):137-140.
[11] 皮灿辉.重组防御素的开发应用[J].北方牧业,2010(24):25.
[12] 张辉华,曹永长,毕英佐.鸡β-防御cDNA的克隆与序列分析[J].中国预防兽医学报,2004,26(3):185-187.
[13] SELSTED M E,OUELLETTE A J.Mammalian defensins in the antimicrobial immune response[J].Nature Immunology,2005,6:551-557.
[14] YANG D,BIRAGYN A,HOOVER D M,et al.Multiple roles of antimicrobial defensins,cathelicidins,and eosinophil-derived neurotoxin in host defense[J].Annual Review of Immunology,2004,22:181-215.
[15] PORTER E.Distinct defensin profiles in Neisseria gonorrhoeae and chlamydia trachomatis urethritis reveal novel epithelial cell-neutrophil interactions[J].Infection and Immunity,2005,73:4823-4833.
[16] KOUGIAS P.Defensins and cathelicidins:neutrophil peptides with roles in inflammation,hyperlipidemia and atherosclerosis[J].Journal of Molecular Cell Biology,2005,9:3-10.