The aim of this study was to observe the effects of red clover (Trifolium pretense) isoflavones on several blood endogenous hormone levels of broilers. Two hundreds and forty 1-day-old AA broilers were randomly divided into 4 groups with 4 replicates per group and 15 broilers per replicate. The broilers in the 4 groups were fed basal diet supplemented with 0 (control group), 10 (experimental groupⅠ), 20 (experimental group Ⅱ) and 30 mg/kg (experimental group Ⅲ) red clover isoflavones, respectively. The whole experiment period was 7 weeks. The results showed as follows: the daily weight gain of broilers in the experimental groups Ⅰand Ⅱ was significantly higher than that in the control group and experimental group Ⅲ (P<0.05 or P<0.01). Feed/gain in the experimental groups was all lower than that in the control group, but had no significant difference (P<0.05). As for serum growth hormone (GH) level, the experimental group Ⅱ was significantly higher than the control group at 21, 35 and 49 days of age (P<0.05 or P<0.01); the experimental group Ⅰ at 49 days of age and experimental group Ⅲ at 21 days of age was higher than the control group (P<0.05 or P<0.01). Serum insulin-like growth factor-Ⅰ (IGF-Ⅰ) level in the experimental groups was all higher than that in the control group at 21 days of age (P<0.05), and that in the experimental group Ⅱ was significantly higher than that in the control group and other experimental groups at 49 days of age (P<0.05). Serum triiodothyronine (T3) level in the experimental group Ⅰ at 35 and 49 days of age and experimental group Ⅱ at 49 days of age was higher than that in the control group (P<0.05 or P<0.01), and serum thyroxine (T4) level in the experimental groups Ⅰ and Ⅲ at 35 days of age was lower than that in the control group (P<0.05). Serum T3/T4 in experimental groups was all higher than that in the control group at 21, 35 and 49 days of age, and experimental group Ⅰ had significant difference at 35 and 49 days of age (P<0.05). Red clover isoflavones had no significant effect on serum testosterone (TESTO) level of broilers (P>0.05). In conclusion, supplementation of red clover isoflavones can improve growth performance by regulating the levels of GH, IGF-Ⅰ,T3 and T4 in broilers, and the supplementation of 20 mg/kg has the better effect.
JIANG Yibao
,
YANG Yurong
,
CUI Guowen
,
WANG Chenzhang
. Effects of Red Clover (Trifolium pratense) Isoflavones on Several Blood Endogenous Hormone Levels of Broilers[J]. Chinese Journal of Animal Nutrition, 2012
, 24(6)
: 1137
-1142
.
DOI: 10.3969/j.issn.1006-267x.2012.06.021
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