Feed Restriction and Realimentation Affect Activities of Hepatic Key Enzymes in Mongolia Growing Wethers

XU Yongxia; HOU Xianzhi; WANG Hairong; GAO Aiwu; LI Junliang; YANG Jinli

doi:10.3969/j.issn.1006-267x.2012.07.008

Chinese Journal of Animal Nutrition >

2012 , Vol. 24 >Issue 7: 1236 - 1245

DOI: https://doi.org/10.3969/j.issn.1006-267x.2012.07.008

RUMINANT NUTRITION

Feed Restriction and Realimentation Affect Activities of Hepatic Key Enzymes in Mongolia Growing Wethers

XU Yongxia ,
HOU Xianzhi ,
WANG Hairong ,
GAO Aiwu ,
LI Junliang ,
YANG Jinli

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1. College of Animal Science, Inner Mongolia Agricultural University, Hohhot 010018, China;
2. College of Food Science and Engineering, Inner Mongolia Agricultural University, Hohhot 010018, China

Received date: 2012-01-07

Online published: 2012-07-05

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Abstract

This experiment was conducted to study the effects of feed restriction and realimentation on the activities of hepatic key enzymes in Mongolia growing wethers. Forty three-month-old growing wethers with an average body weight of (14.72±1.10) kg were selected and divided into 2 groups (control group and restricted group). The feeding trial included a 60-d feed restriction period and a 90-d feed realimentation period. The metabolizable energy (ME) and crude protein (CP) content in diets were 8.62 MJ/kg and 5.7% for restricted group and were 10.88 MJ/kg and 15.0% for the control group during the feed restriction period. All growing wethers were offered the same diet (ME: 9.75 MJ/kg; CP: 12%) during the feed realimentation period. At the end of feed restriction and realimentation period, the activities of hexokinase (HK), pyruvate kinase (PK), lactate dehydrogenase (LDH), fructose-1,6-bisphosphatase (FBP1), malate dehydrogenase (MDH), α-glycerophosphate dehydrogenase (GAPDH), alanine aminotransferase (ALT), aspartate aminotransferase (AST) and glutamate dehydrogenase (GLDH) in liver in each group were examined. The results showed that the activities of hepatic HK, ALT, AST, GLDH of restricted group were significantly lower than those of the control group (P<0.05) at the end of feed restriction period, while the activities of PK, LDH, FBP1, MDH and GAPDH of restricted group were significantly higher than those of the control group (P<0.05). At the end of realimentation period, the activities of hepatic HK, PK, MDH and ALT of restricted group were significantly higher than those of the control group (P<0.05), while the activities of LDH, FBP1, GAPDH, AST and GLDH showed no significant differences (P>0.05). These results indicate that the activities of key enzymes in the glycolysis and protein metabolism are decreased, and the activities of key enzymes in gluconeogenesis and lipolysis are increased. That may be related to the strengthening of fat mobilization during the feed restriction period. The activities of key enzymes in protein metabolism are decreased which means to decrease protein metabolism during the feed restriction period. At the end of realimentation period, the activities of key enzymes in glycolysis, gluconeogenesis and lipid metabolism are recovered to the level of control group, while the activities of hepatic PK and key enzymes in fat synthesis and protein metabolism of control group are significantly higher, this may be related to the higher growth rate during the restriction period.

Key words： feed restriction; realimentation; Mongolia growing wethers; liver; key enzyme activities

Cite this article

XU Yongxia , HOU Xianzhi , WANG Hairong , GAO Aiwu , LI Junliang , YANG Jinli . Feed Restriction and Realimentation Affect Activities of Hepatic Key Enzymes in Mongolia Growing Wethers[J]. Chinese Journal of Animal Nutrition, 2012 , 24(7) : 1236 -1245 . DOI: 10.3969/j.issn.1006-267x.2012.07.008

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