FISH NUTRITION

Utilization Effects of Crystalline Amino Acids and Requirement of Lysine for Juvenile Yellowcheek Carp (Elopichthys bambusa)

  • YANG Wei ,
  • FAN Qixue ,
  • ZONG Kejin ,
  • SONG Lin ,
  • ZHANG Yunlong ,
  • PENG Cong ,
  • HU Peipei
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  • College of Fisheries, Huazhong Agricultural University, Wuhan 430070, China

Received date: 2012-02-01

  Online published: 2012-07-05

Abstract

An 8-week feeding trial was conducted to evaluate the utilization effects of crystalline amino acids for juvenile yellowcheek carp (Elopichthys bambusa), and to determine the dietary lysine requirement. Seven isonitrogenous diets were formulated. The diet which had conjugated proteins (fish meal and casein) as the only protein source was control group diet. In the diets of experimental groups, crystalline amino acids were used to replace part of casein on the basis of the control group diet, and different levels of L-lysine稨Cl were supplemented into the above diets to achieve lysine levels from 1.9% to 4.5% with a 0.5% increment. A total of 378 juvenile yellowcheek carp with initial body weight of (2.36?0.08) g were randomly divided into 7 groups with 3 replicates per group and 18 fish per replicate, and each group were randomly fed one of the 7 experimental diets. The results showed as follows: although growth performance appeared different degrees of decline when part of casein replaced by crystalline amino acids, the special gain rate of 3.46% and 3.94% groups had no significant difference compared with the control group (P<0.05), and feed conversation ratio of 2.45%, 2.95%, 3.46% and 3.94% groups had no significant difference compared with the control group (P<0.05). The apparent digestibility of dry matter, protein and lipid of experimental groups were significantly higher than those of control group (P>0.05). The weight gain rate, special gain rate, apparent digestibility of dry matter and protein, hepatopancreas lipase activity, serum protein and cholesterol contents, and serum alkaline phosphatase activity of juvenile yellowcheek carp had a trend first increased and then decreased with increasing dietary lysine level. It is concluded that crystalline amino acids can be used by juvenile yellowcheek carp, and the utilization effects are related to the balance of amino acid composition. Using weight gain rate as index, quadratic curve model indicate that the optimal dietary lysine requirement for juvenile yellowcheek carp is 3.40% (DM basis) when dietary crude protein content is 46% (DM basis).

Cite this article

YANG Wei , FAN Qixue , ZONG Kejin , SONG Lin , ZHANG Yunlong , PENG Cong , HU Peipei . Utilization Effects of Crystalline Amino Acids and Requirement of Lysine for Juvenile Yellowcheek Carp (Elopichthys bambusa)[J]. Chinese Journal of Animal Nutrition, 2012 , 24(7) : 1255 -1263 . DOI: 10.3969/j.issn.1006-267x.2012.07.010

References

[1] HALVER J E,HARDY R W.Fish nutrition[M].3th ed.Maryland Heights:Elsevier Science,2002.
[2] 冷向军,李小勤,陈丙爱,等.鱼类对晶体氨基酸利用的研究进展[J].水生生物学报,2009,31(1):119-123.
[3] KAUSHIK S J,SEILIEZ I.Protein and amino acid nutrition and metabolism in fish:current knowledge and future needs[J].Aquaculture Research,2010,41:322-332.
[4] 宓国强,沈土山,许谷星,等.鳡的人工繁殖与胚胎发育[J].水产学报,2007(5):639-646.
[5] 樊启学,杜海明,朱邦科,等.鳡人工繁殖试验[J].水利渔业,2007(2):47-48.
[6] 樊启学,杜海明,朱邦科,等.鳡鱼苗种池塘培育技术[J].水利渔业,2007(3):32-33.
[7] 杜海明,刘文奎,张磊,等.投喂频率对鳡幼鱼摄食及生长的影响[J].淡水渔业,2007(6):15-18.
[8] 周华,樊启学,宗克金,等.饲料碳水化合物水平对鳡幼鱼生长和体成分的影响[J].水生态学杂志,2011,32(3):108-113.
[9] 杨磊.蛋白质水平对鳡幼鱼生长、消化和蛋白质代谢的影响.硕士学位论文.武汉:华中农业大学,2011.
[10] 赵巧娥,朱邦科,沈凡,等.饲料脂肪水平对鳡幼鱼生长、体成分及血清生化指标的影响[J].华中农业大学学报:自然科学版,2012,31(3):357-363.
[11] 马徐发,王卫民,杨紫兰.鳡的生化组成与营养特性[J].华中农业大学学报:自然科学版,2008,27(6):759-762.
[12] 万松良,汪亮,李杰,等.鳡含肉率和肌肉营养成分分析[J].淡水渔业,2008(1):27-29.
[13] 李爱杰.水产动物营养与饲料学[M].北京:中国农业出版社,1996:199-200.
[14] WEBB K A,Jr.,GATLIN D M.Effects of dietary protein level and form on production characteristics and ammonia excretion of red drum Sciaenops ocellatus[J].Aquaculture,2003,225:17-26.
[15] PERES H,OLIVA-TELES A.The effect of dietary protein replacement by crystalline amino acid on growth and nitrogen utilization of turbot Scophthalmus maximus juveniles[J].Aquaculture,2005,250:755-764.
[16] LUO Z,LIU Y J,MAI K S,et al.Quantitative L-lysine requirement of juvenile grouper Epinephelus coioides[J].Aquaculture Nutrition,2006,12:165-172.
[17] 谭芳芳,叶元土,肖顺应,等.补充微囊赖氨酸和蛋氨酸对草鱼生长性能的影响[J].动物营养学报,2010,22(3):804-810.
[18] ZHOU F,SHAO J,XU R,et al.Quantitative L-lysine requirement of juvenile black sea bream (Sparus macrocephalus)[J].Aquaculture Nutrition,2010,16:194-204.
[19] ZHOU Q C,WU Z H,CHI S Y,et al.Dietary lysine requirement of juvenile cobia (Rachycentron canadum)[J].Aquaculture,2007,273:634-640.
[20] AHMED I,KHAN M A.Dietary lysine requirement of fingerling Indian major carp, Cirrhinus mrigala (Hamilton)[J].Aquaculture,2004,235:499-511.
[21] 周贤君,解绶启,谢从新,等.异育银鲫幼鱼对饲料中赖氨酸的利用及需要量研究[J].水生生物学报,2006,30(3):247-255.
[22] 杜强,林黑着,牛津,等.卵形鲳鲹幼鱼的赖氨酸需求量[J].动物营养学报,2011,23(10):1725-1732.
[23] BICUDO Á J A,SADO R Y,CYRINO J E P.Dietary lysine requirement of juvenile pacu Piaractus mesopotamicus (Holmberg, 1887)[J].Aquaculture,2009,297:151-156.
[24] YANG S D,LIU F G,LIOU C H.Assessment of dietary lysine requirement for silver perch (Bidyanus bidyanus) juveniles[J].Aquaculture,2011,312:102-108.
[25] WANG S,LIU Y J,TIAN L X,et al.Quantitative dietary lysine requirement of juvenile grass carp Ctenopharyngodon idella[J].Aquaculture,2005,249:419-429.
[26] RUCHIMAT T,MASUMOTO T,HOSOKAWA H,et al.Quantitative lysine requirement of yellowtail (Seriola quinqueradiata)[J].Aquaculture,1997,158:331-339.
[27] 宋理平,韩勃,王爱英,等.碳水化合物水平对厚唇弱棘鯻生长和血液指标的影响[J].大连海洋大学学报,2010,25(4):293-297.
[28] 王宏田,徐永立,张培军.假雄牙鲆消化器官中碱性磷酸酶比活性的研究[J].海洋科学集刊,2001,43:157-160.
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