In this experiment, small intestinal digestibility of rumen undegraded protein (RUP) in 13 kinds of concentrates were measured by mobile nylon bag method (MBT), original three-step in vitro method (TSP), modified three-step in vitro method (MTSP) and acid detergent insoluble nitrogen (ADIN) estimation method, and correlation analysis of measured results between MBT and other methods and regression analysis between conventional nutrient contents and small intestinal digestibility of RUP were carried out. The purpose of this experiment was to find out a simple way to replace the MBT to determine small intestinal digestibility of RUP. Three cows with permanent rumen fistulas and duodenal T-fistulas were chosen and single-factor experimental design was selected in this experiment. Test materials were soybean meal, cottonseed meal, rapeseed meal, sunflower meal, sesame meal, corn germ meal, corn, rice bran, rice bran cake, rice bran meal, barley, wheat bran, wheat bran, corn fiber feed. The results showed that small intestinal digestibility of RUP measured by MBT were 98.13%, 87.37%, 88.47%, 82.60%, 85.73%, 75.40%, 93.23%, 69.27%, 77.83%, 92.10%, 91.27%, 72.37% and 79.03% in soybean meal, cottonseed meal, rapeseed meal, sunflower meal, sesame meal, corn germ meal, corn, rice bran, rice bran meal, rice bran bread, barley, wheat bran and corn fiber feed, respectively. Correlation coefficient of measured results between MTSP and MBT was 0.838 3, correlation coefficient of measured results between TSP and MBT was 0.789 9, and there was no significant correlation of measured results between MBT and ADIN estimation method (P>0.05). The small intestinal digestibility of RUP was significantly correlated with conventional nutrient contents (P<0.05). The effect of small intestinal digestibility of feed RUP measured by MTSP replacing MBT was better than that measured by TSP replacing MBT. The effect of small intestinal digestibility of protein feed RUP measured by in vitro method replacing MBT was better than that of energy feed. The small intestinal digestibility of RUP can be predicted by feed conventional nutrient contents.
WANG Yan
,
XIN Hangshu
,
YANG Fang
,
CHEN Changdong
,
ZHANG Weiwei
,
LI Min
,
XIA Ke
,
ZHANG Yonggen
. Small Intestinal Digestibility of Rumen Undegraded Protein Measured by Different Methods and Correlation Analysis[J]. Chinese Journal of Animal Nutrition, 2012
, 24(7)
: 1264
-1273
.
DOI: 10.3969/j.issn.1006-267x.2012.07.011
[1] CALSAMIGLIA S,STERN M D.A three-step in vitro procedure for estimating intestinal digestion of protein in ruminants[J].Journal of Animal Science,1995,73:1459-1462.
[2] TAGHIZADEH A,MESGARAN M D,VALIZADEH R.Digestion of feed amino acids in the rumen and intestine of steers measured using a mobile nylon bag technique[J].Journal of Dairy Science,2005,88:1807-1814.
[3] HARSTAD O M,PRESTLKKEN E.Effective rumen degradability and intestinal indigestibility of individual amino acids in solvent extracted soybean meal (SBM) and xylose treated SBM (SoyPass) determined in situ[J].Animal Feed Science and Technology,2000,83:31-47.
[4] 岳群,杨红建,谢春元,等.应用尼龙袋法和三步法评定反刍动物家畜常用饲料的蛋白质小肠消化率[J].中国农业大学学报,2007,12(6):62-66.
[5] 赵青余.移动尼龙袋法和三步体外法测定反刍动物常用饲料蛋白质小肠消化率的研究.博士学位论文.北京:中国农业科学院,2005.
[6] NRC.Nutrient requirements of dairy cattle[S].7th ed.Washington,D.C.:National Academy Press,2001.
[7] KUSUMANTI E,WEISBJERG M R,HVELPLUND T.A comparison between protein disappearance from the mobile bag and acid detergent solubility of nitrogen as estimates of protein digestibility in ruminants[J].Animal Feed Science and Technology,1996,5:337-345.
[8] ALDERMAN G,COTTRILL B R.Energy and protein requirements of ruminants:an advisory manual prepared by the AFRC technical committee on responses to nutrients[M].Wallingford:CAB International,1993.
[9] WEBSTER A J F,KITCHERSIDE M A,KEIRBY J R,et al.Hall evaluation of protein feeds for dairy cows[J].Journal of Animal Production,1984,8:548-553.
[10] GARGALLO S,CALSAMIGLIA S,FERRET A.Technical note:a modified three-step in vitro procedure to determine intestinal digestion of proteins[J].Journal of Animal Science,2006,84:2163-2167.
[11] HVELPLUND T.Digestibility of rumen microbial protein and undegraded dietary protein estimated in the small intestine of sheep and by in sacco procedure[J].Acta Agriculturae Scandinavica.Supplementum,1985,25:132-144.
[12] WOODS V B,MONLONEY A P,et al.The nutritive value of concentrate feedstuffs for ruminant.Part Ⅲ.Small intestinal digestibility as measured by in vitro or mobile bag techniques[J].Animal Feed Science and Technology,2003,110:145-157.
[13] CHALUPA W,SNIFFEN C J.Protein and amino acid nutrition of lactating dairy cattle-today and tomorrow[J].Animal Feed Science and Technology,1996,58(1/2):65-75.
[14] ANTONIEWICZ A M,VANVURREN A M,VAN DER KOELEN C J.Intestinal digestibility of rumen undegraded protein of formaldehyde-treated feedstuffs measured by mobile bag and in vitro technique[J].Animal Feed Science and Technology,1992,39:111-124.
[15] VAN STRAALEN W M,DOOPER F M H,ANTONIEWICZ A M,et al.Intestinal digestibility in dairy cows of protein from grass and clover measured with mobile bag and other methods[J].Journal of Dairy Science,1993,76:297-298.
[16] NAKAMUR A T,KLOPFENSTEIN T J,GIBB D J,et al.Growth efficiency and digestibility of heated proteins fed to growing ruminants[J].Journal of Animal Science,1994,72:774-782.
[17] ROGERS J A,CONRAD H R,DEHORITY B A,et al.Microbial numbers,rumen fermentation,and nitrogen utilization of steers fed wet or dried brewers grains[J].Journal of Dairy Science,1986,69:745-753.
