EXPERIMENTAL METHODS

Predicting Utilizable Crude Protein Content in Small Intestine by Cornell Net Carbohydrate and Protein System

  • WANG Yan ,
  • YANG Fang ,
  • CHEN Changdong ,
  • WANG Haiwei ,
  • ZHANG Weiwei ,
  • ZHANG Yonggen
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  • 1. College of Animal Science and Technology, Northeast Agriculture University, Harbin 150030, China;
    2. Qiqihar University, Qiqihar 161006, China;
    3. Hebei Normal University of Science and Technology, Qinhuangdao 066000, China;
    4. College of Life Science and Technology, Northeast Agriculture University, Harbin 150030, China

Received date: 2012-02-01

  Online published: 2012-07-05

Abstract

This study was conducted to establish mathematical models of predicting utilizable crude protein (uCP) content in feed for dairy cows using Cornell net carbohydrate and protein system (CNCPS). Contents of protein fractions of 13 kinds of common feeds were analyzed using CNCPS, and uCP of feeds was determined using mobile nylon bag method, and three Holstein dairy cows with permanent ruminal and duodenal cannulas were used in a single factor experimental design. The results showed as follows: 1) contents of uCP in soybean meal, cottonseed meal, rapeseed meal, sunflower seed meal, sesame meal, corn germ meal, rice bran, rice bran cake, rice bran meal, barley, wheat bran, corn and corn gluten feed were 390.32, 321.90, 297.21, 230.50, 388.62, 177.49, 85.53, 116.78, 134.74, 80.47, 128.26, 70.28 and 66.65 g/kg, respectively. 2) The regression equation of contents of measured uCP and CNCPS fractions in all experimental feeds was uCP=-4.11+6.48PA+7.73PB1+5.72PB2+8.26PB3+5.11PC (R2=0.997 2, P<0.01); the regression equation of contents of measured uCP and CNCPS fractions in protein feed was uCP=12.79+5.47PA+7.04PB1+9.74PB2+8.14PB3 (R2=0.998 7,P<0.01); the regression equation of contents of measured uCP and CNCPS fractions in energy feed was uCP=14.80+8.55PA+6.27PB2+17.64PB3 (R2=0.987 4, P<0.01). 3) Regression coefficients (R2) between CNCPS fractions contents and measured uCP contents in protein feed, energy feed and all experimental feed were all higher than 0.95, and regression coefficient between measured uCP content and CNCPS fractions contents in protein feed was higher than that in energy feed. In conclusion, it is feasible that using contents of protein components subdivided by CNCPS to predict uCP content, and the prediction result in protein feed is more accurate than that in energy feed.

Cite this article

WANG Yan , YANG Fang , CHEN Changdong , WANG Haiwei , ZHANG Weiwei , ZHANG Yonggen . Predicting Utilizable Crude Protein Content in Small Intestine by Cornell Net Carbohydrate and Protein System[J]. Chinese Journal of Animal Nutrition, 2012 , 24(7) : 1274 -1282 . DOI: 10.3969/j.issn.1006-267x.2012.07.012

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