Different Selenium Sources and Levels Affect Growth Performance, Serum Antioxidant Ability and Tissue Selenium Retention in Wistar Rats

LUO Peilin; ZHENG Ping; HE Jun; MAO Xiangbing; YU Bing; CHEN Daiwen

doi:10.3969/j.issn.1006-267x.2012.07.016

2012 , Vol. 24 >Issue 7: 1311 - 1319

DOI: https://doi.org/10.3969/j.issn.1006-267x.2012.07.016

FEED SCIENCE AND TECHNOLOGY

Different Selenium Sources and Levels Affect Growth Performance, Serum Antioxidant Ability and Tissue Selenium Retention in Wistar Rats

LUO Peilin ,
ZHENG Ping ,
HE Jun ,
MAO Xiangbing ,
YU Bing ,
CHEN Daiwen

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Key Laboratory for Animal Disease-Resistance Nutrition of Ministry of Education, Animal Nutrition Institute, Sichuan Agricultural University, Ya’an 625014, China

Received date: 2012-02-01

Online published: 2012-07-05

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Abstract

This experiment was conducted to investigate the effects of different selenium (Se) sources and levels on growth performance, serum antioxidant ability and tissue Se retention in Wistar rats, and to assess the bioavailability value of Se-yeast A produced using molasses and urea as carbon source and nitrogen source in our laboratory, compared with sodium selenite and commercial Se-yeast (Se-yeast B). A total of 70 Wistar rats aged about 8 weeks after two-week Se-depletion experiment were weighted and allotted to 10 treatments with 7 replicates per treatment and 1 rat per replicate. The rats were fed one of the 10 diets (a basal diet, the basal diet with 0.3 mg/kg Se from Se-yeast B, the basal diet with 0.1, 0.2, 0.3 and 0.40 mg/kg Se from Se-yeast A or sodium selenite) for 21 days. The results showed as follows: 1) Se sources and levels did not influence growth performance, serum glutathione peroxidase (GSH-Px) and superoxide dismutase (SOD) activities (P>0.05); 2) tissue Se content was increased linearly as the dietary Se level increased (P<0.01), but the increase was markedly higher when Se-yeast A was fed (P<0.05); 3) according to slope ratio assay, the bioavailability values of Se-yeast A were 95.9%, 127.5%, 114.5% and 101.2% compared with sodium selenite, when serum GSH-Px activity and Se content in liver, kidney and muscle were used as indicators, respectively; 4) there were no significant differences on growth performance, serum antioxidant ability in rats between Se-yeast A and Se-yeast B (P>0.05), but rats fed Se-yeast A had more Se retention in kidney and less Se retention in muscle than those fed Se-yeast B (P<0.01). These results indicate that different Se sources have great effects on the deposition of tissue Se, and Se-yeast has higher bioavailability value than sodium selenite, but there are some differences between Se-yeast A and Se-yeast B; different Se levels have less influence on antioxidant ability, if serum GSH-Px activity is used as standard, then 0.1 mg/kg Se-yeast or sodium selenite can meet the needs of the rats, but higher level of Se-yeast has more tissue Se reserve.

Key words： sodium selenite; yeast selenium; GSH-Px; tissue selenium content; bioavailability value

Cite this article

LUO Peilin , ZHENG Ping , HE Jun , MAO Xiangbing , YU Bing , CHEN Daiwen . Different Selenium Sources and Levels Affect Growth Performance, Serum Antioxidant Ability and Tissue Selenium Retention in Wistar Rats[J]. Chinese Journal of Animal Nutrition, 2012 , 24(7) : 1311 -1319 . DOI: 10.3969/j.issn.1006-267x.2012.07.016

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