γ-Aminobutyric Acid Affects Serum Cytokine Contents and mRNA Expression Levels of Bcl-2 and Fas genes in Spleen of Chicks under Beak Trimming Stress

XIE Wanying; HOU Xinyan; YAN Fengbin; HAN Ruili; SUN Guirong; KANG Xiangtao

doi:10.3969/j.issn.1006-267x.2012.07.021

2012 , Vol. 24 >Issue 7: 1352 - 1360

DOI: https://doi.org/10.3969/j.issn.1006-267x.2012.07.021

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γ-Aminobutyric Acid Affects Serum Cytokine Contents and mRNA Expression Levels of Bcl-2 and Fas genes in Spleen of Chicks under Beak Trimming Stress

XIE Wanying ,
HOU Xinyan ,
YAN Fengbin ,
HAN Ruili ,
SUN Guirong ,
KANG Xiangtao

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College of Livestock Husbandry and Veterinary Engineering, Henan Agricultural University, Henan Innovative Engineering Research Center of Poultry Germplasm Resource, Zhengzhou 450002, China

Received date: 2012-02-01

Online published: 2012-07-05

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Abstract

This experiment was conducted to investigate the effects of γ-aminobutyric acid (GABA) on growth performance, serum cytokine contents and mRNA expression levels of apoptosis-related gene B-cell lympoma/leukemia-2 (Bcl-2) and Fas gene in spleen of chicks under beak trimming stress. A total of 360 one-day-old healthy Gu-shi male chicks were randomly divided into 5 groups (A, B, C, D and E) with 6 replicates per group and 12 chicks per replicate. Groups A, B and C were supplemented with 40, 60 and 80 mg/kg GABA in drinking water, group D was not supplemented with GABA, and group E was supplemented with GABA. The results showed as follows: 1) beak trimming induced FI and BW to decrease, whereas supplemented with GABA could increased FI and BW of chicks under beak trimming stress (P<0.05). 2) The contents of interleukin-1β (IL-1β), interleukin-6 (IL-6)and tumor necrosis factor-α (TNF-α)in serum were increased by beak trimming stress, the serum IL-1β content in group E was significantly higher than that in group D on the first and fifth days (P<0.05), and the supplementation of 80 mg/kg GABA significantly decreased the serum IL-1β content. Compared with group D, the contents of TNF-α and IL-6 in serum in group E were significantly increased on the first and seventh days (P<0.05), and supplementation of 80 mg/kg GABA significantly decreased the contents of TNF-α and IL-6 in serum (P<0.05). 3) The splenetic Fas gene mRNA expression level in group E was significantly higher than that in group D on the first, third and seventh days (P<0.05), and the splenetic Fas gene mRNA expression level was decreased with GABA supplementation (P<0.05), the supplementation of 80 mg/kg GABA could increase the splenetic Bcl-2 gene mRNA expression level (P>0.05). In conclusion, GABA can improve the growth performance of chick under beak trimming stress, and it also inhibit serum cytokines contents and splenetic Fas gene mRNA expression under beak trimming stress, and the optimum supplemention level of GABA is 80 mg/kg.

Key words： γ-aminobutyric acid; beak trimming stress; cytokine; apoptosis; chicks

Cite this article

XIE Wanying , HOU Xinyan , YAN Fengbin , HAN Ruili , SUN Guirong , KANG Xiangtao . γ-Aminobutyric Acid Affects Serum Cytokine Contents and mRNA Expression Levels of Bcl-2 and Fas genes in Spleen of Chicks under Beak Trimming Stress[J]. Chinese Journal of Animal Nutrition, 2012 , 24(7) : 1352 -1360 . DOI: 10.3969/j.issn.1006-267x.2012.07.021

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