Long-Chain n-3 Polyunsaturated Fatty Acids Affect mRNA Expression of Pro-Inflammatory Cytokine Genes in Intestinal Epithelial Cells

HUANG Zhiqing; CHEN Xiaoling; CHEN Daiwen; YU Bing; HE Jun

doi:10.3969/j.issn.1006-267x.2012.07.025

2012 , Vol. 24 >Issue 7: 1384 - 1388

DOI: https://doi.org/10.3969/j.issn.1006-267x.2012.07.025

SHORT COMMUNICATIONS

Long-Chain n-3 Polyunsaturated Fatty Acids Affect mRNA Expression of Pro-Inflammatory Cytokine Genes in Intestinal Epithelial Cells

HUANG Zhiqing ,
CHEN Xiaoling ,
CHEN Daiwen ,
YU Bing ,
HE Jun

Expand

Key Laboratory for Animal Disease-Resistance Nutrition of China Ministry of Education, Institute of Animal Nutrition, Sichuan Agricultural University, Ya’an 625014, China

Received date: 2012-03-22

Online published: 2012-07-05

Fold

Abstract

This present study was conducted to investigate the effects of long-chain n-3 polyunsaturated fatty acids (LC n-3 PUFA) on the mRNA expression of pro-inflammatory cytokine genes in intestinal epithelial cells. IEC-6 cells from rat intestinal epithelial cells were used as the experimental model and subjected to four treatments with three replicates in each treatment and one well per replicate. The treatments were as follows: control, lipopolysaccharide (LPS, 1 μg/mL), LPS(1 μg/mL)+docosahexaenoic acid (DHA, 100 μmol/L) and LPS (1 μg/mL)+eicosapentaenoic acid (EPA,100 μmol/L). IEC-6 cells was collected for total RNA extraction after 48 h pretreatment with DHA, EPA or equivalent amounts of dimethyl sulfoxide (solvent of DHA and EPA, control) and 3 h treatment with LPS. The mRNA expression levels of tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β) and interleukin-6 (IL-6) genes were determined using real-time quantitative PCR. The results showed that the mRNA expression levels of TNF-α, IL-1β and IL-6 genes were significantly up-regulated after LPS challenge (P<0.01), EPA significantly attenuated LPS-induced upregulation of mRNA expression levels of TNF-α (P<0.01), IL-1β (P<0.01) and IL-6 genes (P<0.05), while DHA only significantly attenuated LPS-induced upregulation of mRNA expression level of IL-1β gene (P<0.05). These results indicate that LC n-3 PUFA has an anti-inflammatory effect in intestinal epithelial cells, and the effect of EPA is more efficient than that of DHA under conditions in this experiment.

Key words： long-chain n-3 polyunsaturated fatty acid; eicosapentaenoic acid; docosahexaenoic acid; IEC-6 cells; pro-inflammatory cytokines

Cite this article

HUANG Zhiqing , CHEN Xiaoling , CHEN Daiwen , YU Bing , HE Jun . Long-Chain n-3 Polyunsaturated Fatty Acids Affect mRNA Expression of Pro-Inflammatory Cytokine Genes in Intestinal Epithelial Cells[J]. Chinese Journal of Animal Nutrition, 2012 , 24(7) : 1384 -1388 . DOI: 10.3969/j.issn.1006-267x.2012.07.025

References

[1] MAYER K,MEYER S,REINHOLZ M M,et al.Short-time infusion of fish oil based lipid emulsions,approved for parenteral nutrition,reduces monocyte proinflammatory cytokine generation and adhesive interaction with endothelium in humans[J].Journal of Immunology,2003,171(9):4837-4843.
[2] WELDON S M,MULLEN A C,LOSCHER C E,et al.Docosahexaenoic acid induces an anti-inflammatory profile in lipopolysaccharide-stimulated human THP-1 macrophages more effectively than eicosapentaenoic acid[J].Journal of Nutritional Biochemistry,2007,18(4):250-258.

[3] MARCHESELLI V L,HONG S,LUKIW W J,et al.Novel docosanoids inhibit brain ischemia-reperfusion-mediated leukocyte infiltration and pro-inflammatory gene expression[J].Journal of Biological Chemistry,2003,278(44):43807-43817.

[4] MOON D O,KIM K C,JIN C Y,et al.Inhibitory effects of eicosapentaenoic acid on lipopolysaccharide-induced activation in BV2 microglia[J].International Immunopharmacology,2007,7(2):222-229.

[5] SAEDISOMEOLIA A,WOOD L G,GARG M L,et al.Anti-inflammatory effects of long-chain n-3 PUFA in rhinovirus-infected cultured airway epithelial cells[J].British Journal of Nutrition,2009,101(4):533-540.

[6] 史艳莉,李翠娥,代方梅,等.n-3多不饱和脂肪酸对运动性哮喘豚鼠气道炎症和气道高反应性的影响[J].中国运动医学杂志,2010,29(6):669-672,687.
[7] LIU F,LI G,WEN K,et al.Porcine small intestinal epithelial cell line (IPEC-J2) of rotavirus infection as a new model for the study of innate immune responses to rotaviruses and probiotics[J].Viral Immunology,2010,23(2):135-149.

[8] PFAFFL M W.A new mathematical model for relative quantification in real-time RT-PCR[J].Nucleic Acids Research,2001,29:45.
[9] QUARONI A,WANDS J,TRELSTAD R L,et al.Epithelioid cell cultures from rat small intestine:characterization by morphologic and immunological criteria[J].Journal of Cell Biology,1979,80(2):248-265.

[10] ARCE C,RAMÍREZ-BOO M,LUCENA C,et al.Innate immune activation of swine intestinal epithelial cell lines (IPEC-J2 and IPI-2I) in response to LPS from Salmonella typhimurium[J].Comparative Immunology Microbiology and Infectious Diseases,2010,33(2):161-174.

[11] LYU S Y,PARK W B.Mistletoe lectin modulates intestinal epithelial cell-derived cytokines and B cell IgA secretion[J].Archives of Pharmacal Research,2009,32(3):443-451.

[12] 蔡景义,周安国,田刚,等.氧化锌对LPS处理猪肠上皮细胞炎性细胞因子分泌及基因表达的影响[J].中国畜牧杂志,2011,47(23):47-50.
[13] CAUGHEY G E,MANTZIORIS E,GIBSON R A,et al.The effect on human tumor necrosis factor α and interleukin 1β production of diets enriched in n-3 fatty acids from vegetable oil or fish oil[J].American Journal of Clinical Nutrition,1996,63(1):116-122.
[14] CLELAND L G,JAMES M J,PROUDMAN S M.Dietary fats and inflammation:the medicinal use of fish oil[J].Nutrition & Dietetics,2009,66(1):4-6.

[15] HALADE G V,WILLIAMS P J,LINDSEY M L,et al.Fish oil decreases inflammation and reduces cardiac remodeling in rosiglitazone treated aging mice[J].Pharmacological Research,2011,63(4):300-307.

[16] MONK J M,KIM W,CALLAWAY E,et al.Immunomodulatory action of dietary fish oil and targeted deletion of intestinal epithelial cell PPAR delta in inflammation-induced colon carcinogenesis[J].American Journal of Physiology:Gastrointestinal and Liver Physiology,2012,302(1):G153-G167.
[17] KEW S,MESA M D,TRICON S,et al.Effects of oils rich in eicosapentaenoic and docosahexaenoic acids on immune cell composition and function in healthy humans[J].American Journal of Clinical Nutrition,2004,79(4):674-681.
[18] ALEXANDER J W.Immunonutrition:the role of N-3 fatty acids[J].Nutrition,1998,14(7/8):627-633.

Options

Outlines

模态框（Modal）标题

Abstract

Cite this article

References