Effects of Glutamine on Growth Performance, Apparent Digestibility of Nutrients, Jejunal Alkaline Phosphatase Activity and Expression of Genes Related to Intestinal Health in Weaner Piglets

XIAO Yingping; HONG Qihua; LIU Xiuting; LIU Ruigang; ZHAO Xuke; CHEN Anguo; YANG Caimei

doi:10.3969/j.issn.1006-267x.2012.08.007

2012 , Vol. 24 >Issue 8: 1438 - 1446

DOI: https://doi.org/10.3969/j.issn.1006-267x.2012.08.007

Molecular Nutrition

Effects of Glutamine on Growth Performance, Apparent Digestibility of Nutrients, Jejunal Alkaline Phosphatase Activity and Expression of Genes Related to Intestinal Health in Weaner Piglets

XIAO Yingping ,
HONG Qihua ,
LIU Xiuting ,
LIU Ruigang ,
ZHAO Xuke ,
CHEN Anguo ,
YANG Caimei

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College of Animal Sciences, Zhejiang University, Hangzhou 310058, China

Received date: 2012-02-24

Online published: 2012-08-03

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Abstract

The aim of the present paper was to provide a profile of the effects of Gln on growth performance, apparent digestibility of nutrients, jejunal alkaline phosphatase activity and expression of genes related to intestinal health in weaner piglets. One hundred and twenty-eight 21-day-old piglets (Duroc×Landrace×Yorkshire) were randomly assigned into control group and 1.00% Gln group according to their body weight and sex, and each group contained 4 replicates and 16 piglets per replicate. The fresh feces samples were collected in each replicate on days 10 and 30 post-weaning, and one piglet was selected for jejunum sample collection based on its weight near to the average weight of the corresponding group. The experiment lasted for 30 days. Results showed that average daily gain, the apparent digestibility of dry matter (DM), crude protein (CP), and apparent metabolizable energy (AME) in the Gln group were increased by 19.05%, 9.06%, 4.77% and 7.02%(P＜0.05)compared with the control group on day 10 post-weaning, and the apparent digestibility of most amino acids in the Gln group were also increased (P＜0.05); the apparent digestibility of DM, CP, and AME were increased by 5.90%, 2.80% and 6.43%(P＜0.05) on day 30 post-weaning. Jejunal brush border membrane-bound alkaline phosphatase activity in the Gln group was increased by 30.09% (P＜0.05) and 5.98% (P＞0.05) on days 10 and 30 post-weaning, respectively. Peroxisome proliferator-activated receptor gamma (PPAR-γ) and pyruvate kinase (PK) mRNA levels were decreased by 10.00% (P＞0.05) and 30.00% (P＞0.05) on day 10 post-weaning, and by 41.88% and 44.40% (P＜0.05) on day 30 post-weaning. Gln supplementation increased the mammalian target of rapamycin (mTOR) mRNA levels by 22.00% (P＞0.05) and 22.38% (P＜0.05) on days 10 and 30 post-weaning. These data indicate that Gln increases jejunal alkaline phosphatase activity and expression of genes related to intestinal health, which improves growth performance and apparent digestibility of nutrients in weaner piglets.

Key words： glutamine; weaner piglets; apparent digestibility; alkaline phosphatase; gene expression

Cite this article

XIAO Yingping , HONG Qihua , LIU Xiuting , LIU Ruigang , ZHAO Xuke , CHEN Anguo , YANG Caimei . Effects of Glutamine on Growth Performance, Apparent Digestibility of Nutrients, Jejunal Alkaline Phosphatase Activity and Expression of Genes Related to Intestinal Health in Weaner Piglets[J]. Chinese Journal of Animal Nutrition, 2012 , 24(8) : 1438 -1446 . DOI: 10.3969/j.issn.1006-267x.2012.08.007

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