Protective Effect of Vitamin C on Oxidative Damage in Intestinal Epithelial Cells of Jian Carp (Cyprinus carpio var. Jian)

LIU Yang; CHI Lei; FENG Lin; JIANG Jun; ZHOU Xiaoqiu

doi:10.3969/j.issn.1006-267x.2012.08.014

2012 , Vol. 24 >Issue 8: 1503 - 1511

DOI: https://doi.org/10.3969/j.issn.1006-267x.2012.08.014

Molecular Nutrition

Protective Effect of Vitamin C on Oxidative Damage in Intestinal Epithelial Cells of Jian Carp (Cyprinus carpio var. Jian)

LIU Yang ,
CHI Lei ,
FENG Lin ,
JIANG Jun ,
ZHOU Xiaoqiu

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1. Animal Nutrition Institute, Sichuan Agricultural University, Ya’an 625014, China;
2. Fish Nutrition and Safety Production University Key Laboratory of Sichuan Province, Ya’an 625014, China;
3. Key Laboratory for Animal Disease-Resistance Nutriton of China Ministy of Education, Ya’an 625014, China;
4. Chengdu Sunwind Agricaltural and Livestack Farming Co. , Ltd. , Xinjin 621430, China

Received date: 2012-05-14

Online published: 2012-08-03

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Abstract

This experiment was conducted to investigate the protective effect of different concentrations of vitamin C on hydrogen peroxide (H₂O₂)-induced oxidative damage in intestinal epithelial cells (IEC) of Jian carp. In 24-well culture plates, the Jian carp IEC were primarily cultured for 72 h, and then continued to culture in serum-free DMEM culture medium. After 12 h further culture, the IEC were randomly divided into 7 groups with 4 replicates per group. One of the 7 groups was set as blank control, and the other groups were all supplemented with 100 μmol/L H₂O₂ in culture medium in order to establish oxidative stress model. After 16 h further culture in the same condition, the culture medium was changed into the DMEM culture medium which contained 0 (blank control group), 0, 4, 10, 16, 22 and 28 mg/L vitamin C, respectively. The samples were collected to measure the indices after 72 h vitamin C culture. The results showed as follows: 1) the 100 μmol/L H₂O₂ treatment led to less survival number and small colony of IEC; significantly decreased the activities of superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPX), glutathione S-transferase (GST) and glutathion reductase (GR), and reduced the contents of glutathione (GSH) and protein (P<0.05); significantly increased the contents of malonaldehyde (MDA) and protein carbonyl (PCA), and improved the lactate dehydrogenase (LDH) activity in culture medium (P<0.05); caused oxidative stress of Jian carp IEC. 2) Vitamin C significantly increased the anti-hydroxyl free radical ability (AHA) and anti-superoxide anion free radical ability (ASA) (P<0.05), and recovered the oxidation damage in IEC of Jian carp induced by H₂O₂. In summary, vitamin C can improve the antioxidant ability of the Jian carp IEC, and effectively protect the IEC from oxidative damage.

Key words： Jian carp; vitamin C; IEC; antioxidant ability

Cite this article

LIU Yang , CHI Lei , FENG Lin , JIANG Jun , ZHOU Xiaoqiu . Protective Effect of Vitamin C on Oxidative Damage in Intestinal Epithelial Cells of Jian Carp (Cyprinus carpio var. Jian)[J]. Chinese Journal of Animal Nutrition, 2012 , 24(8) : 1503 -1511 . DOI: 10.3969/j.issn.1006-267x.2012.08.014

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