This experiment was conducted to study the effects of dietary vitamin D3 level on expression of antimicrobial peptide hepcidin gene in Monopterus albus. A total of 360 healthy M. albus with an average body weight of (21.72?2.1) g was randomly assigned to 6 groups with 2 replicates per group and 30 M. albus per replicate. The M. albus in the 6 groups were fed the basal diet (0 IU/kg vitamin D3) supplemented with 0, 250, 500, 1 000, 2 000 and 4 000 IU/kg vitamin D3, respectively. The hepatopancreas, head kidney, spleen and hindgut in each group were collected on the 20th, 40th and 60th days. The expression of hepcidin gene in each sample were analyzed using real-time PCR. Results showed as follows: after feeding 20, 40 and 60 days, the hepcidin gene had expressed in 4 tissues of M. albus, and that in hepatopancreas was higher than that in head kidney, spleen and hindgut (P<0.05). At the same feeding stage, with increasing vitamin D3 supplemental level, the expression of hepcidin gene in 4 tissues was firstly increased and then decreased. On the 20th day, the highest values of hepcidin gene expression in 4 tissues were all found in 2 000 IU/kg group, and there were significant differences between 2 000 IU/kg group and other groups (P<0.05). On the 40th and 60th days, the highest values of hepcidin gene expression in hepatopancreas and head kidney were all found in 500 IU/kg group, and there were significant differences between 500 IU/kg group and 0, 250, 4 000 IU/kg groups on the 40th day (P<0.05), and between 500 IU/kg group and other groups on the 60th day (P<0.05). In conclusion, in a short time (20 days) feeding, supplementation of 2 000 IU/kg vitamin D3 can significantly promote the hepcidin gene expression in splanchnic tissues of M. albus; in a long time (40 or 60 days) feeding, supplementation of 500 IU/kg vitamin D3 can significantly promote the hepcidin gene expression in hepatopancreas and head kidney of M. albus.
LI Debing
,
LI Chao
,
ZHANG Gongwei
,
TANG Yong
,
ZHOU Dinggang
,
LI Xuewei
. Effects of Dietary Vitamin D3 Level on Expression of Antimicrobial Peptide hepcidin Gene in Monopterus albus[J]. Chinese Journal of Animal Nutrition, 2012
, 24(8)
: 1520
-1526
.
DOI: 10.3969/j.issn.1006-267x.2012.08.016
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