The objective of this trial was to study the effects of mixed volatile fatty acid (VFA) sodium salts on activities of digestive enzymes in intestinal digesta and nitrogen (N) retention in lambs. Fifteen cross-bred male lambs, aged 7 weeks, with an average body weight of (13.8±0.8) kg, were randomly divided into five groups with three lambs in each group. Lambs were all fed milk replacers which provided basal nutrient requirement for them. The supplemental level of mixed VFA sodium salts (molar proportion of acetate, propionate and butyrate was 65∶25∶10) in each group was 0, 5, 15, 30 and 60 g/d, respectively. The feeding trial lasted for 24 days. At the last four days of the feeding trial, faeces and urine samples were collected for the analysis of faecal N and urinary N. Lambs were slaughtered to collect intestinal digesta for the analysis of pH and activities of α-amylase, trypsin, lipase, chymotrypsin and galactohydrolase. The results showed as follows: no significant difference was found among groups in pH and activities of trypsin, lipase, chymotrypsin and galactohydrolase in intestinal digesta (P>0.05), the same as faecal N, urinary N and N apparent digestibility (P>0.05). Supplementation of mixed VFA sodium salts at 30 and 60 g/d significantly decreased the α-amylase activity (P<0.05), and tended to increase the N retention (P=0.090). It is concluded that supplementation of mixed VFA sodium salts at 30 to 60 g/d can decrease the α-amylase activity, and tends to increase the N retention, whereas activities of intestinal trypsin, lipase, chymotrypsin and galactohydrolase will not be affected.
DING Xihong
,
ZHAO Guangyong
. Effects of Mixed Volatile Fatty Acid Sodium Salts on Activities of Intestinal Digestive Enzymes and Nitrogen Retention in Lambs[J]. Chinese Journal of Animal Nutrition, 2012
, 24(8)
: 1543
-1547
.
DOI: 10.3969/j.issn.1006-267x.2012.08.019
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