Effects of Licorice Extracts on Rumen Fermentation and Methane Yield of Sheep in Vitro

GUO Xuefeng; LIU Junfeng; SUN Libin; GAO Jun; ZAHNG Sujiang

doi:10.3969/j.issn.1006-267x.2012.08.020

Chinese Journal of Animal Nutrition >

2012 , Vol. 24 >Issue 8: 1548 - 1556

DOI: https://doi.org/10.3969/j.issn.1006-267x.2012.08.020

Molecular Nutrition

Effects of Licorice Extracts on Rumen Fermentation and Methane Yield of Sheep in Vitro

GUO Xuefeng ,
LIU Junfeng ,
SUN Libin ,
GAO Jun ,
ZAHNG Sujiang

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1. Key Laboratory of Tarim Animal Husbandry Science & Technology, Alar 843300, China;
2. College of Animal Science, Tarim University, Alar 843300, China

Received date: 2012-02-29

Online published: 2012-08-03

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Abstract

This study was conducted to study the effects of licorice extracts on rumen fermentation and methane yield of sheep in vitro. Rumen fluid was collected from three Karakul sheep with permanent rumen fistulas. The screening test of supplemental level of licorice extracts was done by in vitro gas production method. The substrate was the basal diet of sheep, and was incubated with licorice extracts at levels of 0 (control group), 0.5%, 1.0%, 3.0%, 5.0% and 7.0 for 6, 9, 12, 15, 18, 21 and 24 h, respectively. Rumen fermentation parameters, including gas production, culture medium pH, volatile fatty acid concentration, ammonia nitrogen (NH₃-N) concentration and methane yield, were observed, and the effects were assessed by multiply factor analyses of index (MFAEI). The results showed as follows: after 24 h fermentation, gas production of groups 5.0% and 7.0% was significantly lower than that of the control group (P<0.05); culture medium pH and NH₃-N concentration were not significantly affected (P>0.05); concentrations of acetic acid and propionic acid (except for group 5.0% at 21 h) of experimental groups were lower than that of the control group; after 24 h fermentation, methane yield of group 7.0% was significantly lower than that of the control group; MFAEI showed group 1.0%>group 0.5%>group 7.0%>group 3.0%>group 5.0%. The results indicate that 1.0% is the proper supplemental level of licorice extract under conditions in this study.

Key words： licorice extracts; rumen fermentation; methane; Karakul sheep

Cite this article

GUO Xuefeng , LIU Junfeng , SUN Libin , GAO Jun , ZAHNG Sujiang . Effects of Licorice Extracts on Rumen Fermentation and Methane Yield of Sheep in Vitro[J]. Chinese Journal of Animal Nutrition, 2012 , 24(8) : 1548 -1556 . DOI: 10.3969/j.issn.1006-267x.2012.08.020

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