This experiment was conducted to study the effects of silage corn seeds on performance, egg quality and intestinal environment in laying hens. Two hundred 55-week-old Hy-Line laying hens were randomly divided into 4 groups with 5 replicates per group and 10 hens per replicate. The laying hens in the 4 groups were fed the basal diet with 0 (control group), 1%, 2% and 4% silage corn seeds, respectively. The experiment lasted for seven weeks. The results showed as follows: compared with the control group, laying rate, average daily egg production, eggshell color, Haugh unit and the number of Lactobacillus in jejunum and caecum of laying hens fed the diets containing 2% and 4% silage corn seeds were increased significantly (P<0.05), while feed to egg ratio and the number of Escherichia coli in jejunum and caecum were decreased significantly (P<0.05). Average daily egg production, Haugh unit, the number of Lactobacillus in jejunum and caecum of laying hens fed the diets containing 1% silage corn seeds were increased significantly (P<0.05), while the number of Escherichia coli in jejunum and caecum was decreased significantly (P<0.05). pH of jejunum and caecum of laying hens fed the diets containing 4% silage corn seeds was decreased significantly (P<0.05). It is concluded that silage corn seeds can improve performance, egg quality and intestinal environment of laying hens, and the addition of 2% silage corn seeds to the diet is ideal.