In order to evaluate the relation of different stress conditions and concentrations of swine blood acute phase proteins (APPs), three simulated patterns in swine production were used. Thirty-nine pigs injected swine Pasteurella multocida vaccine (PMV), swine Japanese encephalitis vaccine (SJEV) and lipopolysaccharide (LPS) had imitated bacterial infection, virus infection and inflammatory, respectively. The behavior, haematological parameters and the concentrations of APPs were recorded followed by stress processing. Pigs injected LPS vomited yellow liquid, had a fever, and felt short of breath. Numbers of neutrophile (NEUT) and monocyte (MO) in blood were increased significantly (P<0.05) after 48 h of treatment by PMV. Numbers of NEUT and MO were increased significantly (P<0.05) after 24 or 48 h of treatment by LPS, respectively. Contents of hemoglobin (HGB) and numbers of platelet (PLT) were decreased significantly (P<0.05) after 48 or 24 h of treatment by SJEV, respectively. Numbers of red blood cell (RBC), PLT and content of HGB were decreased significantly (P<0.05) after 24 and 48 h of treatment by LPS. The concentration of haptoglobin (HPT) was increased significantly (P<0.05) after 48 h of treatment by PMV, and after 72 h of treatment by SJEV. The concentration of major acute phase protein (MAP) was decreased significantly (P<0.05) after 72 h of treatment by PMV, and after 24, 48 and 72 h of treatment by LPS. The concentration of MAP was increased significantly (P<0.05) after 24 h of treatment by SJEV. The concentration of apolipoprotein-A1 (Apo-A1) was decreased significantly (P<0.05) after 72 h of treatment by PMV, after 48 h by SJEV, and after 24, 48 and 72 h of treatment by LPS. Positive APP (HPT) was increases significantly in pattern of bacterial or virus infection, while negative APP (Apo-A1) was decreases significantly under the 3 kinds of stress conditions in this study. It preliminarily proves that the concentrations of HPT and Apo-A1 in blood can be used to evaluate swinery health.
QI Keke
,
LI Yongming
,
XU Meifang
,
LV Jinhui
,
XU Ziwei
. Different Stress Treatments Affect Haematological Parameters and Concentrations of Serum Acute Phase Proteins of Pigs[J]. Chinese Journal of Animal Nutrition, 2012
, 24(9)
: 1656
-1663
.
DOI: 10.3969/j.issn.1006-267x.2012.09.007
[1] MOSHAGE H.Cytokines and the hepatic acute phase response[J].The Journal of Pathology,1997,181(3):257-266.
[2] HEINRICH P C,CASTELL T A,ANDUS T.Interleukin-6 and the acute phase response[J].Biochemical Journal,1990,265(3):621-636.
[3] PIÑEIRO C,PIÑEIRO M,MORALES J,et al.Pig-MAP and haptoglobin concentration reference values in swine from commercial farms[J].Veterinary Journal,2009,179(1):78-84.
[4] HEEGAARD P M H,KLAUSEN J,NIELSEN J P,et al.The porcine acute phase response to infection with Actinobacillus pleuropneumoniae. Haptoglobin, C-reactive protein, major acute phase protein and serum amyloid A protein are sensitive indicators of infection[J].Comparative Biochemistry and Physiology,1998,119(2):365-373.
[5] SORENSEN N S,TEGTMEIER C,ANDRESEN L O,et al.The porcine acute phase protein response to acute clinical and subclinical experimental infection with Streptococcus suis[J].Veterinary Immunology and Immunopathology,2006,113(1/2):157-168.
[6] YOSHIOKA M,WATANABE A,SHIMADA N,et al.Regulation of haptoglobin secretion by recombinant bovine cytokines in primary cultured bovine hepatocytes[J].Domestic Animal Endocrinology,2002,23(3):425-433.
[7] SKINNER J G,ROBERTS L.Haptoglobin as an indicator of infection in sheep[J].Veterinary Record,1994,134(1):33-38.
[8] HEEGAARD P M H,STOCKMARR A,PIÑEIRO M,et al.Optimal combinations of acute phase proteins for monitoring disease in pigs[J].Veterinary Immunology and Immunopathology,2009,128:211-347.
[9] 罗满林.猪细胞巨化病毒感染研究进展[J].中国兽医杂志,2004,40(8):34-37.
[10] JOHNSON R,VON BORELL E.Lipopolysaccharide-induced sickness behavior in pigs is inhibited by pretreatment with indomethacin[J].Journal of Animal Science,1994,72(2):309-314.
[11] 黎文彬.酵母硒对脂多糖(LPS)诱导的免疫应激早期断奶仔猪的影响 硕士学位论文.雅安:四川农业大学,2009:27.
[12] CLAPPERTON M,BISHOP S C,CAMERON N D,et al.Associations of weight gain and food intake with leukocyte sub-sets in Large White pigs[J].Livestock Production Science,2005,96(2/3):249-260.
[13] CLAPPERTON M,BISHOP S C,CAMERON N D,et al.Associations of acute phase protein levels with growth performance and with selection for growth performance in Large White pigs[J].Society of Animal Science,2005,81(2):213-220.
[14] CLAPPERTON M,BISHOP S C,PIÑEIRO M,et al.The association between plasma levels of acute phase proteins, haptoglobin, alpha-1 acid glycoprotein (AGP), pig-MAP, transthyretin and serum amyloid A (SAA) in Large White and Meishan pigs[J].Veterinary Immunology and Immunopathology,2007,119(3/4):303-309.
[15] LEVY A P,ASLEH R,BLUM S,et al.Haptoglobin: basic and clinical aspects[J].Antioxidants & Redox Signaling,2010,12(2):293-304.
[16] MINERT V.Serum haptoglobin concentration in growing swine after intranasal challenge with Bordetella bronchiseptica and toxigenic Pasteurella multocida type D[J].Canadian Journal of Veterinary Research,1996,60(3):222-227.
[17] YOSHINO K,KATOH N,TAKAHASHI K,et al.Possible involvement of protein kinase C with induction of haptoglobin in cows by treatment with dexamethasone and by starvation[J].American Journal of Veterinary Research,1993,54(5):689-694.
[18] UCHIDA E,KATOH N,TAKAHASHI K.Appearance of haptoglobin in serum from cows at parturition[J].Journal of Veterinary Medical Science,1993,55(5):893-894.
[19] MURATA H,MIYAMOTO T.Bovine haptoglobin as a possible immunomodulator in the sera of transported calves[J].British Veterinary Journal,1993,149(3):277-283.
[20] LIPPERHEIDE C,RABE M,KNURA S,et al.Effects of farm hygiene on blood chemical variables in fattening pigs[J].Tierärztliche Umschau,2000,55(1):30-36.
[21] MARTIN M,TEESOURO M A,GONZLEZ-RAMN N,et al.Major plasma proteins in pig serum during postnatal development[J].Reproduction Fertility and Development,2005,17(4):439-445.
[22] WRIGHT K J,BALAJI R,HILL C M,et al.Integrated adrenal, somatotropic, and immune responses of growing pigs to treatment with lipopolysaccharide[J].Journal of Animal Science,2000,78(7):1892-1899.
[23] CARPINTERO R,PIÑEIRO M,ANDRÉS M,et al.The concentration of Apolipoprotein A-Ⅰ decreases during experimentally induced acute phase processes in pigs[J].Infection and Immunity,2005,73(5):3184-3187.
[24] CARPINTERO R,ALONSO C,PIÑEIRO M,et al.Pig Major acute phase protein and apolipoprotein A-Ⅰ responses correlate with the clinical course of experimentally induced African Swine Fever and Aujeszky’s disease[J].Veterinary Research,2007,8(5):741-753.
[25] MURATA H,SHIMADA N,YOSHIOKA M.Current research on acute phase proteins in veterinary diagnosis: an overview[J].The Veterinary Journal,2004,168(1):28-40.
