This study was conducted to determine apparent ileal digestible tryptophan (Trp) requirement of female broilers aged 1 to 21 days and effects of dietary tryptophan level on meat quality, antioxidant and immune indices. A total of 480 one-day-old Cobb female broilers were randomly allocated into 5 treatments with 8 replicates per treatment and 12 broilers per replicate. Corn-soybean meal-corn gluten meal diets containing 0.170%, 0.191%, 0.213%, 0.234% and 0.248% Trp (0.170% basis Trp and 0, 0.021%, 0.042%, 0.063% and 0.084% crystal L-Trp, respectively) were provided. Growth performance, meat quality, antioxidant and immune indices of broilers aged 21 days were determined. Apparent digestibility and apparent ileal digestibility of amino acids for broilers aged 21 to 25 days were measured by indicator method. The results showed as follows: dietary Trp level significantly affected feed intake and weight gain of broilers aged 1 to 21 days (P<0.05). There was a quadratic relationship between feed intake or weight gain and dietary apparent ileal digestible Trp level (P<0.05). Based on the response in weight gain, apparent ileal digestible Trp requirement of female broilers aged 1 to 21 days was 0.176% (apparent ileal digestible Trp intake was 68.15 mg/d). Malondialdehyde (MDA) content in breast muscle, total superoxide dismutase (T-SOD) activity in liver and glutamate-pyruvate transaminase activity in serum of broilers fed the diet containing 0.213% or 0.248% Trp were significantly decreased compared with those fed the diet containing 0.170% Trp (P<0.05). Breast muscle pH value at 45 min post-slaughter, intestinal total secretary immunoglobulin A (sIgA) and serum total IgA concentrations of broilers fed the diet containing 0.248% Trp were significantly improved compared with those fed the diet containing 0.170% Trp (P<0.05). The current study suggests that female broilers aged 1 to 21 days need 0.176% (68.15 mg/d) apparent ileal digestible Trp to get the maximum weight gain. Increasing dietary Trp level can alleviate lipid peroxidation and breast muscle acidity post-slaughter, and improve intestinal mucosal immunity and humoral immunity of broilers.
WANG Bo
,
MIN Zhizhi
,
WANG Yongwei
,
WANG Ning
,
HUANG Chunxi
,
YUAN Jianmin
,
GUO Yuming
. Apparent Ileal Digestible Tryptophan Requirement of Female Broilers Aged 1 to 21 Days[J]. Chinese Journal of Animal Nutrition, 2012
, 24(9)
: 1664
-1673
.
DOI: 10.3969/j.issn.1006-267x.2012.09.008
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