This experiment was performed to investigate rumen microbial degradation of phytate phosphorus (PP) in rice, maize, and wheat bran feeds by in vitro ruminal batch culture method. Ruminal fluid was collected from 3 rumen-cannulated Holstein cows at the lactation stage, and PP disappearance rate of those grain bran feeds were determined after 3, 12, 24, 48 and 72 h anaerobic incubation with the rumen fluid. The results showed as follows: 1) PP disappearance rate of those grain bran feeds depended on the incubation time. The longer incubation time was, the more PP was hydrolyzed. When the incubation lasted up to 24 h, nearly 90% of PP in the grain bran feeds was hydrolyzed. 2) Within the same grain bran, differences in the potential degradability and degradation rate of PP were also observed between different sources. 3) Consequently, the potential degradability of PP was up to 100% in wheat bran (P<0.000 1), and no significant difference occurred between maize bran (95.58%) and rice bran (96.82%) (P>0.05). The degradation rate of PP in the grain bran feeds was ranked as: rice>maize>wheat. In summary, mixed rumen microorganisms have high ability to hydrolyze PP in grain bran feeds, though there is a difference in degradation of different grain bran feeds.
SHEN Shasha
,
YANG Hongjian
,
ZHANG Zhihui
,
WEN Ya
,
REN Qingchang
,
ZHANG Xiaoming
. A Comparison Study of in Vitro Degradation Characteristic of Phytate Phosphorus in Rice, Maize and Wheat Bran Feeds by Mixed Rumen Microorganisms[J]. Chinese Journal of Animal Nutrition, 2012
, 24(9)
: 1707
-1712
.
DOI: 10.3969/j.issn.1006-267x.2012.09.013
[1] SHARPLEY A N,CHAPRA S C,WEDEPOHL R,et al.Managing agricultural phosphorus for protection of surface waters:issues and options[J].Journal of Environmental Quality,1994,23(3):437-451.
[2] 沈莎莎,杨红建,张晓明.瘤胃微生物植酸酶的分类与酶学性质研究进展[J].畜牧兽医学报,2011,42(12):1655-1660.
[3] MESCHY F,RAMIREZ-PEREZ A H.Recent developments of dietary phosphorus recommendations[J].Productions Animales,2005,18(3):175-182.
[4] 李胜利,张胜利,刘建新,等.奶牛产业与技术进展[J].中国畜牧杂志,2011,47(4):42-46.
[5] MENKE K H,STEINGASS H.Estimation of the energetic feed value obtained from chemical analysis and in vitro gas production using rumen fluid[J].Animal Research and Development,1988,28:7-55.
[6] 王丽.不同来源植酸酶对常用饲料中植酸盐降解效率的研究.硕士学位论文.南京:南京农业大学,2007.
[7] ØRSKOV E R,MCDONALD I.The estimation of protein degradability in the rumen from incubation measurements weighted according to rate of passage[J].Journal of Agricultural Science,1979,92(2):499-503.
[8] 张丽英.饲料分析及饲料质量检测技术[M].3版.北京:中国农业大学出版社,2007.
[9] HAUG W,LANTZSCH H J.Sensitive method for the rapid determination of phytate in cereals and cereal products[J].Journal of the Science of Food and Agriculture,1983,34(12):1423-1426.
[10] RAUN A,CHENG E,BURROUGHS W,et al.Ruminant nutrition:phytate phosphorus hydrolysis and availability to rumen microorganisms[J].Journal of Agricultural and Food Chemistry,1956,4(10):869-871.
[11] BRASK-PEDERSEN D N,GLITSO L V,SKOV L K,et al.Effect of exogenous phytase on feed inositol phosphate hydrolysis in an in vitro rumen fluid buffer system[J].Journal of Dairy Science,2011,94(2):951-959.
[12] TILLMAN A D,BRETHOUR J R.Utilization of phytin phosphorus by sheep[J].Journal of Animal Science,1958,17(1):104-112.
[13] ELLIS L C,TILLMAN A D.Utilisation of phytin phosphorus in wheat bran by sheep[J].Journal of Animal Science,1961,20:606-607.
[14] CLARK W D,WOHLT J E,GILBREATH R L,et al.Phytate phosphorus intake and disappearance in the gastrointestinal tract of high producing dairy cows[J].Journal of Dairy Science,1986,9(12):3151-3155.
[15] REID R L,FRANKLIN M C,HALLSWORTH E G,et al.The utilization of phytate phosphorus by sheep[J].Australian Veterinary Journal,1947,23(6):136-140.
[16] KINCAID R L,GARIKIPATI D K,NENNICH T D,et al.Effect of grain source and exogenous phytase on phosphorus digestibility in dairy cows[J].Journal of Dairy Science,2005,88(8):2893-2902.