This study was conducted to investigate the effects of N-acetylcysteine (NAC) on intestinal mucosal immune stress of piglets single challenged with lipopolysaccharide (LPS). Twenty-four healthy piglets were randomly allocated into 4 groups with 6 replicates and 1 piglet per replicate. Piglets in the control and LPS groups were fed a basal diet, and piglets in the 250 and 500 mg/kg NAC groups were fed the basal diet supplemented with 250 and 500 mg/kg NAC, respectively. On day 17, piglets in the LPS, 250 and 500 mg/kg NAC groups were injected intraperitoneally with LPS at a dosage level of 100 μg/kg BW, whereas piglets in the control group were injected intraperitoneally with normal saline at the same volume. At 6 h after the LPS or physiological saline injection, all piglets were slaughter to obtain intestinal mucosa for analysis. The results showed that compared with the control group, LPS challenge increased the levels of mucosal interleukin-2, interleukin-6, prostaglandin E2 and the expression of heat stress protein 70 (HSP70) (P<0.05). However, the supplementation of 250 or 500 mg/kg NAC in the diet reduced the levels of these parameters in intestinal mucosa challenged with LPS (P<0.05). In conclusion, the supplementation of 250 or 500 mg/kg NAC in the diet can prevent the increasing of intestinal inflammatory mediators and alleviate immune stress by LPS challenge.
WU Guohua
,
LI Jiao
,
HOU Yongqing
,
WANG Lei
,
YI Dan
,
DING Bingying
,
ZHU Huiling
,
LIU Yulan
. Effects of N-acetylcysteine on Intestinal Mucosal Immune Stress of Piglets Single Challenged with Lipopolysaccharide[J]. Chinese Journal of Animal Nutrition, 2012
, 24(9)
: 1793
-1798
.
DOI: 10.3969/j.issn.1006-267x.2012.09.024
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