The purpose of this study was to explore the effects of nitrogen-free diets on cecal microflora and endogenous amino acid basal losses of broilers aged 14 and 35 days. A total of 308 Male Ross broiler chickens, aged 10 and 31 days, respectively, were chosen and randomly assigned into two dietary treatments, in which one was fed a conventional diet and the other was fed a nitrogen-free diet for 4 days. Every treatment contained five replicates with 12 chickens on day 10 and 6 chickens on day 31 per replicate. The results showed that endogenous basal losses of most amino acids determined by excreta-based assay were significantly higher than those determined by ileum digesta-based assay in broilers aged 14 and 35 days (P<0.05 or P<0.01). Compared with the conventional diet, there were some changes of DGGE predominant bands of cecal microflora 16S rDNA V3 region in broilers fed the nitrogen-free diet aged 14 and 35 days, the 72% and 39% of total gray value of predominant bands changed respectively for broilers fed the nitrogen-free diet aged 14 and 35 days. On day 14, there was no significant difference in cecal digesta weight and ammoniacal nitrogen concentration in broilers between nitrogen-free diet group and conventional diet group (P>0.05), but the concentrations of butyric acid (P<0.01), isobutyric acid (P<0.05) and valeric acid (P<0.05) in cecum digesta of broilers were significantly decreased and propionic acid concentration was significantly increased (P<0.01) by the nitrogen-free diet compared with the conventional diet. On day 35, compared with conventional diet group, cecal digesta weight of broilers in the nitrogen-free diet group was significantly increased, and the concentrations of ammoniacal nitrogen, acetic acid, isobutyric acid, valeric acid and isovaleric acid were significantly decreased (P<0.01). In conclusion, endogenous basal losses of most amino acids measured by excreta-based assay are higher than those measured by ileal digesta-based assay when broilers are fed the nitrogen-free diet. Some predominant species of cecal microflora should change after broilers are fed the nitrogen-free diet for 4 days.
JIANG Chunwei
,
GUO Yuming
,
WANG Yongwei
,
LEI Ting
,
GUO Shuangshuang
. Nitrogen-Free Diets:Effects on Cecal Microflora and Endogenous Amino Acid Basal Losses of Broilers[J]. Chinese Journal of Animal Nutrition, 2012
, 24(10)
: 1878
-1887
.
DOI: 10.3969/j.issn.1006-267x.2012.10.006
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