Using Caco-2 cells as an in vitro model to investigate the adhesive properties of several strains of lactic acid bacteria and the inhibition effects of these strains on the adhesion of Escherichia coli K88 and S. typhimurium. The adhesion of different stains was evaluated by fluorescence labeling method, and preincubation, postincubation and coincubation tests were used to determine the inhibition to the two strains of pathogenic bacteria above. The results showed as follows:1) except for Lactococcus lactis, the adhesion rate of the other lactic acid bacteria strains was higher than that of the two pathogenic bacteria strains, and the adhesion rate was Lactobacillus>Enterococcus>pathogenic bacteria>Lactococcus. 2) The adhesion rate of Lactobacillus from healthy chicken intestines was closed to that from human intestines (Lactobacillus fructivorans). 3) Most strains of lactic acid bacteria could inhibit the adhesion of Escherichia coli K88 and S. typhimurium to Caco-2 cells through postincubation test. In conclusion, the experimental lactic acid bacteria stains possess high adhesion rate, especially Lactobacillus, and the adhesion has specificity on species. Postincubation is the main way that these strains inhibit adhesion of pathogenic bacteria, and has no specificity on species, mean while there is no certain link with the adhesion capacity of strains.
BAI Jie
,
LI Weifen
,
HUANG Qin
,
CUI Zhiwen
,
YU Dongyou
,
HUANG Yi
. Several Strains of Lactic Acid Bacteria:Adhesion Ability to Caco-2 Cells and Effects on Adhesion of Pathogenic Bacteria[J]. Chinese Journal of Animal Nutrition, 2012
, 24(10)
: 1992
-1998
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DOI: 10.3969/j.issn.1006-267x.2012.10.019
[1] COTTER P A,MILLER J F.Triggering bacterial virulence[J].Science,1996,273:1183-1185.
[2] COLLINS J K,THORNTON G,SULLIVAN G O.Selection of probiotic strains for human applications[J].International Dairy Journal,1998,8:487-490.
[3] 范小兵,周丛,杭晓敏,等.昂立植物乳杆菌黏附肠上皮细胞的研究[J].中国微生态学杂志,2004,16(6):353-355.
[4] 王斌,李秋荣,刘放南,等.1株肠道高黏附性乳杆菌的分离鉴定[J].中国生物制品学杂志,2008,21(6):473-477.
[5] CANDELA M,PERNA F,CARNEVALI P,et al.Interaction of probiotic Lactobacillus and Bifidobacterium strains with human intestinal epithelial cells:adhesion properties,competition against enteropathogens and modulation of IL-8 production[J].International Journal of Food Microbiology,2008,125(3):286-292.
[6] VINDEROLAĆG,MEDICI M,PERDIGN G.Relationship between interaction sites in the gut,hydrophobicity,mucosal immunomodulating capacities and cell wall protein profiles in indigenous and exogenous bacteria[J].Journal of Applied Microbiology,2004,96(2):230-243.
[7] BONAR A,CHMIELA M,RUDNICKA W,et al.Mannose-binding lectin enhances the attachment and phagocytosis of mycobacteria in vitro[J].Archivum Immunologiae et Therapia Experimentalis,2005,53:437-441.
[8] GOPAL P K,PRASAD J,SMART J,et al.In vitro adherence properties of Lactobacillus rhamnosus DR20 and Bifidobacterium lactis DR10 strains and their antagonistic activity against an enterotoxigenic Escherichia coli[J].International Journal of Food Microbiology,2001,67(3):207-216.
[9] 陈臣,郭本恒,陈卫,等.三株益生菌黏附性质及机制的初步研究[J].中国微生态学杂志,2007,19(6):492-494.
[10] NISSEN L,CHINGWARU W,SGORBATI B,et al.Gut health promoting activity of new putative probiotic/protective Lactobacillus spp.strains:a functional study in the small intestinal cell model[J].International Journal of Food Microbiology,2009,135(3):288-294.
[11] 王斌.乳杆菌黏附机制的研究进展[J].国际检验医学杂志,2006,27(3):224-226.
[12] 李平兰,杨华,张篪.乳酸菌体外黏附人结肠腺癌细胞系HT-29细胞的研究[J].中国农业大学学报,2002,7(1):19-22.
[13] GUEIMONDE M,JALONEN L,HE F,et al.Adhesion and competitive inhibition and displacement of human enteropathogens by selected Lactobacilli[J].Food Research International,2006,39(4):467-471.
[14] MATIJAšIĆB B,NARAT M,PETERNEL M Z,et al.Ability of Lactobacillus gasseri K7 to inhibit Escherichia coli adhesion in vitro on Caco-2 cells and ex vivo on pigs'jejunal tissue[J].International Journal of Food Microbiology,2006,107(1):92-96.
[15] 郭彤,胥保华,马玉龙,等.嗜酸乳杆菌和两歧双歧杆菌体外黏附Caco-2细胞及其对病原菌黏附性能的影响[J].中国兽医学报,2008,28(5):527-531.
[16] TUOMOLA E M,OUWEHAND A C,SALMINEN S J.The effect of probiotic bacteria on the adhesion of pathogens to human intestinal mucus[J].FEMS Immunology & Medical Microbiology,1999,26(2):137-142.
[17] COLLADO M C,GUEIMONDE M,HERNNDEZ M,et al.Adhesion of selected Bifidobacterium strains to human intestinal mucus and the role of adhesion in enteropathogen exclusion[J].Journal of Food Protection,2005,68(12):2672-2678.
[18] 陈臣,郭本恒,王荫榆,等.植物乳杆菌ST-Ⅲ黏附肠上皮样细胞系Caco-2性质的研究[J].乳业科学与技术,2008,31(2):51-55.
[19] GRANATO D,BERGONZELLI G E,PRIDMORE R D,et al.Cell surface-associated elongation factor Tu mediates the attachment of Lactobacillus johnsonii NCC533 (La1) to human intestinal cells and mucins[J].Infection and Immunity,2004,72(4):2160-2169.
[20] MANGELL P,LENNERNAS P,WANG M,et al.Adhesive capability of Lactobacillus plantarum 299v is important for preventing bacterial translocation in endotoxemic rats[J].Acta Pathologica,Microbiologica et Immunologica Scandinavica,2006,114(9):611-618.
