To investigate the effects of soybean isoflavones (SI) on testis morphology and expression of marker genes during spermatogenesis of Xiang pigs, 40 healthy male pigs aged 28 days were selected and divided randomly into 4 groups with 10 replicates in each group and 1 pig per replicate. Pigs in groups 1, 2, 3 and 4 were fed a basal diet supplemented with 0, 125, 250 and 500 mg/kg SI, respectively. Five pigs in each group were slaughtered after 60 d of feeding, and the tissue of testis was sampled for the analysis of tissue morphology and gene expression of Cdh1, SCP3, Tnp1 and Vim. The results showed as follows:supplementation of SI (all doses in the study) induced vacuolization in seminiferous tubules, and decreased the number of mature sperm in tubules; the supplementation significantly decreased the expression levels of Cdh1, SCP3 and Tnp1 genes (P<0.01); the expression level of Vim gene was significantly decreased by 250 mg/kg SI (P<0.05). In conclusion, dietary SI at doses of 125 to 500 mg/kg has obvious injury on testis morphology of Xiang pigs, and inhibits expressions of marker genes during spermatogenesis. Dietary SI at a dose of 250 mg/kg inhibits expression of the marker gene of sustentacular cell of testis.
FAN Juexin
,
ZHANG Bin
,
LI Lili
,
YUAN Xiaoxue
,
WANG Shuilian
,
XIE Ting
,
GENG Meimei
. Soybean Isoflavones Affect Testis Morphology and Expression of Marker Genes during Spermatogenesis of Xiang Pigs[J]. Chinese Journal of Animal Nutrition, 2012
, 24(10)
: 2021
-2027
.
DOI: 10.3969/j.issn.1006-267x.2012.10.023
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