The objective of this study was to investigate the changes of expressions of insulin-like growth factor Ⅰ (IGF-Ⅰ) and insulin-like growth factor Ⅰ receptor (IGF-ⅠR) genes in the ruminal epithelium of dairy goats in the process of inducing subacute rumen acidosis (SARA) under the condition of different dietary ratios of non-fiber carbohydrate and neutral detergent fiber (NFC/NDF). This study was designed for four experimental periods with twelve lactating Guanzhong dairy goats, and 15 days in each period. Goats were fed 4 diets with different NFC/NDF (1.02, 1.24, 1.63 and 2.58, respectively). The induction of SARA was conducted by the gradual increase of dietary NFC/NDF, and expressions of IGF-Ⅰ and IGF-ⅠR genes were quantitatively analyzed by quantitative real-time PCR (qRT-PCR). The results showed as follows:expressions of IGF-Ⅰ and IGF-ⅠR genes were both increased with the gradual increase of dietary NFC/NDF. The expression of IGF-Ⅰ gene in periods Ⅱ, Ⅲ and Ⅳ was 1.70, 2.71 and 9.61 times of that in period Ⅰ, respectively, and the expression of IGF-ⅠR gene in periods Ⅱ, Ⅲ and Ⅳ was 1.88, 3.09 and 10.19 times of that in period Ⅰ, respectively. Compared with period Ⅰ, expressions of IGF-Ⅰ and IGF-ⅠR genes significantly were increased when NFC/NDF reache 2.58 (P<0.01). In conclusion, induction of SARA using the method of increasing dietary NFC/NDF can significantly increase expressions of IGF-Ⅰ and IGF-ⅠR genes, and they are increased sharply after SARA occurs.
ZHOU Xiangli
,
LIU Dacheng
,
SUN Ge
,
GAO Min
,
HU Honglian
. Dietary NFC/NDF Affects Expressions of IGF-Ⅰ and IGF-ⅠR Genes in the Ruminal Epithelium of Dairy Goats[J]. Chinese Journal of Animal Nutrition, 2012
, 24(10)
: 2036
-2043
.
DOI: 10.3969/j.issn.1006-267x.2012.10.025
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