The aim of this study was to investigate the effects of Bacillus subtilis B1 on antioxidant function of mice fed a high-fat diet. Forty-five ICR mice with similar weight were randomly divided into three groups, control group (fed a basal diet), high-fat group (fed a high-fat diet) and experimental group , with 3 replicates per group and 5 mice per replicate. The mice were raised for 30 days to observe the effects of Bacillus subtilis B1 on the antioxidant capacity and oxidative stress in their intestinal mucosa, liver and serum. The results showed as follows:1) compared with the control group, intestinal mucosal total antioxidant capacity (T-AOC), liver superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), catalase (CAT) and thioredoxin reductase (TrxR) activities and serum glutathione (GSH) content, GSH-Px and anti-superoxide anion radical (anti-O2-?) activities in the high-fat group were significantly decreased by 28.34% (P<0.01), 22.91% (P<0.05), 37.35% (P<0.01), 47.47% (P<0.01), 36.42% (P<0.05), 30.73% (P<0.01), 47.26% (P<0.01) and 8.02% (P<0.01), respectively, whereas liver 8-hydroxydeoxyguanosine (8-OHdG) content in the high-fat group was remarkably increased by 67.87% (P<0.01). 2) Compared with the high-fat group, intestinal mucosal T-AOC, liver GSH content and CAT activity and serum T-AOC, GSH content, GSH-Px and anti-O2-? activities in the experimental group were significantly increased by 32.44% (P<0.05), 38.27% (P<0.05), 53.01% (P<0.05), 10.72% (P<0.05), 38.01% (P<0.05), 51.98% (P<0.01) and 13.45% (P<0.01), respectively, whereas liver malondialdehyde (MDA) and 8-OHdG contents, and xanthine oxidase (XOD) activity in the experimental group were remarkably reduced by 16.01% (P<0.05), 37.52% (P<0.01) and 16.25% (P<0.05), respectively. It is concluded that the high-fat diet can reduce antioxidant capacity and also cause oxidative stress in the intestinal mucosa, liver and serum of mice, and the supplementation of Bacillus subtilis B1 can enhance the antioxidant function of the mice fed the high-fat diet, reduce oxidative stress in their bodies, and protect their cells from oxidative damage.
XU Xin
,
MAO Yulong
,
LI Yali
,
HU Shenglan
,
WU Hongzhao
,
LI Weifen
. Bacillus subtilis B1 Affects Antioxidant Function of Mice Fed a High-Fat Diet[J]. Chinese Journal of Animal Nutrition, 2012
, 24(10)
: 2067
-2072
.
DOI: 10.3969/j.issn.1006-267x.2012.10.029
[1] VALKO M,LEIBFRITZ D,MONCOL J,et al.Free radicals and antioxidants in normal physiological functions and human disease[J].International Journal of Biochemistry & Cell Biology,2007,39(1):44-84.
[2] SLIM R M,TOBOREK M,WATKINS B A,et al.Susceptibility to hepatic oxidative stress in rabbits fed different animal and plant fats[J].Journal of the American College of Nutrition,1996,15:289-294.
[3] NAPOLI C,MARTIN-PADURA I, DE NIGRIS F,et al.Deletion of the p66Shc longevity gene reduces systemic and tissue oxidative stress,vascular cell apoptosis,and early atherogenesis in mice fed a high-fat diet[J].Proceedings of the National Academy of Sciences of the United States of America,2003,100(4):2112-2116.
[4] 黄俊文,林映才,冯定远,等.益生菌、甘露寡糖对早期断奶仔猪生长、免疫和抗氧化机能的影响[J].动物营养学报,2005,17(4):16-20.
[5] 余东游,毛翔飞,秦艳,等.枯草芽孢杆菌对肉鸡生长性能及抗氧化和免疫功能的影响[J].中国畜牧杂志,2010,46(3):22-25.
[6] 王秋林,王浩毅,王树人.氧化应激状态的评价[J].中国病理生理杂志,2005,21(10):2069-2074.
[7] 朱宇旌,张勇.动物对饲料中脂肪的消化、吸收、代谢及其影响因素[J].畜牧业,1999(6):22-24.
[8] 海春旭.自由基医学[M].西安:第四军医大学出版社,2006:321-324.
[9] DAY C P.Non-alcoholic steatohepatitis (NASH):where are we now and where are we going?[J].Gut,2002,50:585-588.
[10] MEDINA J,FEMANDEX L J,GARCIA L,et al.Approach to the pathogenesis and treatment of nonalcoholic steatohepatitis[J].Diabetes Care,2004,27:2057-2066.
[11] 陈瑗,周玫,刘尚喜,等.脂质过氧化损伤在动脉粥样硬化发生发展中的作用[J].中国科学基金,1995,9(4):43-45.
[12] 李武,施用晖,杨瑞丽,等.硫辛酸对高脂饮食小鼠肠道氧化还原状态及消化吸收功能的影响[J].中国病理生理杂志,2009,25(3):577-580.
[13] 彭海英,于洪波,左中.氧化和抗氧化指标在大鼠高脂饮食所致脂肪肝中的动态变化[J].临床军医杂志,2007,35(5):677-679.
[14] 李安林,施用晖,岳鹏,等.硫辛酸对高脂日粮大鼠脂类代谢和抗氧化能力的影响[J].食品科学,2006,27(5):242-245.
[15] 刘超,张学武.黄芪对高脂血症小鼠血脂及脂质过氧化的影响[J].时珍国医国药,2007,18(7):1648-1649.
[16] MABOUDOU P,MATHIEU D,BACHELET H,et al.Detection of oxidative stress.Interest of GC-MS for malondialdehyde and formaldehyde monitoring[J].Biomed Chromatogr,2002,16:199-202.
[17] WU L L,CHIOUD C C,CHANG P Y,et al.Urinary 8-OHdG:a marker of oxidative stress to DNA and a risk factor for cancer,atherosclerosis and diabetics[J].Clinica Chimica Acta,2004,339(1/2):1-9.
