This experiment was conducted to study the effects of dietary methionine level on serum biochemical indices and antioxidant function of lion head geese aged 28 to 70 days. Seven hundred and twenty 28-day-old healthy lion head geese were randomly divided into 6 groups with 6 replicates per group and 20 geese in each replicate. Geese in the 6 groups were fed experimental diets containing 0.26%, 0.36%, 0.46%, 0.56%, 0.66% and 0.76% methionine, respectively. The experiment lasted for 42 days. The results showed as follows: 1) dietary methionine level had no significant influence on average daily gain and daily feed intake (P>0.05), the ratio of feed to gain in 0.56% and 0.66% methionine level groups was significantly lower than that in 0.26% methionine level group (P<0.05). 2) Dietary methionine level had no significant influence on the contents of total protein, albumin, globulin, uric acid, triglyeride and high density lipoprotein in serum (P>0.05). The serum low density lipoprotein content in 0.76% methionine level group was significantly lower than that in 0.26% methionine level group (P<0.05), but compared with the other groups, that had no significant difference (P>0.05). 3) The serum glutathione peroxidase (GSH-Px) activity in 0.66% methionine level group was significantly higher than that in other groups (P<0.05); the serum glutathione hormone content in 0.66% methionine level group was significantly higher than that in other groups (P<0.05) except 0.76% methionine level group (P>0.05); the serum superoxide dismutase activity in 0.66% methionine level group was significantly higher than that in 0.26% methionine level group (P<0.05); the serum malondialdehyde content in 0.66% methionine level group was significantly lower than that in 0.26% and 0.36% methionine level groups (P<0.05). 4) With the increasing of dietary methionine level, the serum GSH-Px activity was increased quadratically (P<0.05), and the estimated methionine level for lion head geese aged 28 to 70 days was 0.654% when serum GSH-Px activity got maximum value. In conclusion, increasing dietary methionine level can improve the antioxidant function of lion head geese aged 28 to 70 days, and the optimum methionine level is 0.66%.
LIN Zhenping
,
FENG Kailing
,
YE Hui
,
DENG Yuanfan
,
XU Liwen
,
YANG Lin
. Effects of Dietary Methionine Level on Serum Biochemical Indices and Antioxidant Function of Lion Head Geese Aged 28 to 70 Days[J]. Chinese Journal of Animal Nutrition, 2012
, (11)
: 2126
-2132
.
DOI: 10.3969/j.issn.1006-267x.2012.11.009
[1] 唐漾波,欧阳佩珍.过量蛋氨酸对大鼠肝、脑组织脂质过氧化作用的研究[J].营养学报,1994,16(4):362-366.
[2] 霍湘,王安利,杨建梅.含硫氨基酸的抗氧化作用[J].生物学通报,2006,41(4):3-4.
[3] 刘升军,呙于明.饲粮蛋氨酸及赖氨酸对雌性肉仔鸡胴体组成的影响[J].中国畜牧兽医杂志,2001(3):4-6.
[4] 姚元枝,贺建华,简友全,等.雪峰乌骨鸡蛋氨酸需要量的研究[J].湖南农业大学学报,2004,30(2):148-152.
[5] 杨正德,陶玉顺,罗爱平.低蛋白质饲料添加蛋氨酸对促进肉仔鸡生长和抑制腹腔脂肪的效果[J].饲料研究,1996(9):2-3.
[6] 冯文喜,谢振宇.饲料中不同蛋氨酸饲喂肉鹅试验[J].现代畜牧兽医,2006(11):11-12.
[7] MILES R D,FEATHERSTON M R.Uric acid excretion by the chicks as an indicator of dietary protein quality[J].Poultry Science,1976,55:98-102.
[8] 吴维恽,蒋宗勇,林映才,等.肉仔鸡蛋氨酸需要量研究(一)[J].饲料博览,1996,8(5):3-5.
[9] 吴维恽,蒋宗勇,林映才,等.中后期可利用蛋氨酸需求参数研究[J].饲料工业,1997,18(8):17-20.
[10] 程茂基,王昌时,吴承章.饲粮蛋氨酸水平对肉仔鸡的影响[J].中国家禽,2000,22(2):14-15.
[11] 李文立,王宝维,林英庭,等.五龙鹅生长前期饲粮蛋氨酸和赖氨酸适宜水平的研究[J].南京农业大学学报,2004,27(2):68-71.
[12] ELKIN R G,STEWART T S,ROGLER J C.Methionine requiremen to female white Pekin ducklings[J].Poultry Science,1986,65:1771-1776.
[13] 罗清尧,高振川.北京肉仔鸭蛋氨酸和赖氨酸需要量研究[J].动物营养学报,2002,14(3):30-35.
[14] 宁淑芳.银香麻鸡赖氨酸-蛋氨酸及苏氨酸适宜需要量的研究 [D].硕士学位论文.南宁:广西大学,2007:1-2.
[15] 周彦文.合浦鹅赖氨酸、蛋氨酸和苏氨酸适宜需要量的研究 [D].硕士学位论文.南宁:广西大学,2008:15-48.
[16] GRAHAM H.Dietary fiber and the monogastric [C]//Proceedings of the 13th Western Nutrition Conference.Saskatoon:Saskatchewan,1992:16-17.
[17] 高妹娟,刘锡锰,高贵等.谷胱甘肽的抗线粒体脂质过氧化作用[J].生物化学杂志,1997,13(3):287-290.
[18] SASTRE J,PALLARDO F V,VINA J.Glutathione,oxidative stress and aging[J].Age,1996,19(4):129-139.
[19] BAINS J S,SHAW C A.Neurodegenerative disorders in humans:the role of glutathione in oxidative stress-mediated neuronal death[J].Brain Research Reviews,1997,25(3):335-358.
[20] 马森.谷胱甘肽过氧化物酶和谷胱甘肽转硫酶研究进展[J].动物医学进展,2008,29(10):53-56.
[21] 管国芳,孙立群.喉癌及癌劳组织脂质过氧化物及超氧化物歧化酶的研究[J].中国颅底外科杂志,1996(12):144-146.
[22] 王增敏,谭利伟,尹兆正.蛋氨酸对开产蛋鸡脂肪代谢性能的影响及机理研究[J].上海畜牧兽医通讯,2009(1):4-5.
[23] 麻丽坤,谭利伟,卫振,等.饲粮蛋氨酸水平对开产蛋鸡体组织生长和产蛋性能的影响[J].浙江农业科学,2006(5):2-3.
[24] 聂伟.蛋氨酸与蛋氨酸轻基类似物在肉仔鸡和蛋鸡饲粮中应用效果的比较研究 [D].硕士学位论文.北京:中国农业大学,2006.
