This study was conducted to investigate the effects of dietary rumen protected arginine (Arg) and N-carbamylglutamic acid (NCG) on protein synthesis rate in intestinal mucosa of fine-wool sheep. Fifteen weaner borwn Ordos fine-wool lambs were randomly divided into five groups, and lambs were fed a basal diet (group 1,control group), the basal diet+0.15 g/d NCG (group 2), the basal diet+ 0.20 g/d NCG (group 3), the basal diet+1.50 g/d Arg (group 4) and the basal diet+2.00 g/d Arg (group 5), respectively.The feeding lasted for 45 d. After 12 h fasting, lambs were injected a flooding-dose of L-phenylalanine,and then the duodenal mucosa and jejunum mucosa samples were collected after 90 min injection to measure the protein synthesis rate.The results showed that the protein synthesis rate in duodenal mucosa and jejunum mucosa in experimental groups was all higher than that in the control group (P<0.05).Compared with group 1, the protein synthesis rate in groups 3, 4 and 5 was increased more obviously, and group 4 was significantly higher than the other experimental groups (P<0.05), while there was no significant difference between group 3 and group 5 (P>0.05). These results indicate that dietary rumen protected Arg and NCG can increase the protein synthesis rate of intestinal mucosa, and the optimal levels are 1.50 g/d Arg and 0.20 g/d NCG,respectively.
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