The objective of this study was to evaluate the effects of dietary lipid level on growth performance of genetic improvement of farmed tilapia (GIFT, Oreochromis niloticus) and its serum biochemical indices and fatty acid composition under cold stress. Fish with an average body weight of (37.0±1.0) g were fed experimental diets containing 4 different levels (1.95%, 6.19%, 8.03% and 9.95%, respectively) of lipid which were supplemented with 0, 4%, 6% and 8% of soybean oil in basal diet (1.95% lipid) for 8 weeks. Each treatment contained 3 replicates and each replicate contained 25 fish. After feeding experiment, experimental fish were acclimated at 28℃ as control, the water temperature was reduced from 28℃ to 15℃ at a rate of 1℃/h to proceed cold stress experiment. The results showed as follows: 1) the survival rate (SR) and feed conservation rate (FCR) were not significantly influenced by dietary lipid level (P>0.05). The weigh gain rate (WGR) and specific growth rate (SGR) increased significantly (P<0.05) with dietary lipid level increasing until dietary lipid level arrived at 6.19%. 2) Under the cold stress, dietary lipid level had significant influence on serum biochemical indices except glucose (GLU) and triglyceride (TG) levels (P<0.05). With dietary lipid level increasing, the levels of GLU, total protein (TP), TG, cholesterol (CHOL) and low density lipoprotein cholesterol (LDL-C) in serum gradually raised, and the serum high density lipoprotein cholesterol (HDL-C) level showed a rising trend and then gradually descended, while the activities of aspartate aminotransferase (AST) and alanine transarninase (ALT) in serum slightly decreased and then increased. 3) Under the cold stress, the proportion of saturated fatty acid (SFA) in liver showed a descending trend and the proportion of unsaturated fatty acid (UFA) in liver showed a rising trend with dietary lipid level increasing. Dietary lipid level had significant influence on n-3 polyunsaturated fatty acid (PUFA) proportion, n-6 PUFA proportion and n-3/n-6 (P<0.05). In conclusion, the optimal level of dietary lipid is 6.19%, which is beneficial to promote the growth of GIFT and its cold-resistance ability. When dietary lipid level reaches or exceeds 8.03%, the higher transaminase activity may burden the metabolism of liver, which is bad for health and growth of fish.
SHI Guicheng
,
DONG Xiaohui
,
CHEN Gang
,
TAN Beiping
,
CHI Shuyan
,
YANG Qihui
,
LIU Hongyu
. Effects of Dietary Lipid Level on Growth Performance of Genetic Improvement of Farmed Tilapia (GIFT, Oreochromis niloticus) and Its Serum Biochemical Indices and Fatty Acid Composition under Cold Stress[J]. Chinese Journal of Animal Nutrition, 2012
, (11)
: 2154
-2164
.
DOI: 10.3969/j.issn.1006-267x.2012.11.013
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