The aim of this study was to investigate the effects of dietary hainanmycin on rumen microflora of dairy cows by quantitative real-time PCR. Three ruminally cannulated cows were randomly assigned into three treatments in a 3?3 Latin square design. Cows in negative control group were fed a basal diet, and those in positive control group were fed the basal diet supplemented with 350 mg/d monensin, while those in hainanmycin group were fed the basal diet supplemented with 20 mg/d hainanmycin. The results showed as follows: compared with negative control group, contents of Butyrivibrio fibrisolvens, protozoa and fungi in hainanmycin group significantly were decreased by 38.24%, 60.24% and 12.73%, respectively (P<0.01). Contents of Fibrobacter succinogenes, Ruminococcus albus, Ruminococcus flavefaciens and Methanogen were not significantly affected (P>0.05). The content of Methanogen ranged from 0.46% to 0.48%; while the other rumen microorganisms changed with the change of time in different tendencies; in general, contents of rumen microorganisms in hainanmycin and positive control groups varied in the same tend, and the values were close (P>0.05). It is concluded that dietary hainanmycin can significantly alter rumen microflora.
XIN Hangshu
,
DUAN Chunyu
,
ZHANG Yonggen
,
WANG Mingjun
,
LI Zhongyu
,
WANG Zhibo
,
LI Fuguo
. Dietary Hainanmycin Affects Rumen Microflora of Dairy Cows[J]. Chinese Journal of Animal Nutrition, 2012
, (11)
: 2249
-2256
.
DOI: 10.3969/j.issn.1006-267x.2012.11.025
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