This experiment was conducted to study the effects of dietary methionine level on growth performance, digestive and absorptive ability of grass carp (Ctenopharyngodon idell) during middle growth stage, and to determine the methionine requirement of grass carp during middle growth stage. A total of 900 grass carp with an average body weight of (259.00±0.48) g were randomly assigned to 6 treatments with 5 replicates per treatment and 30 fish per replicate. The fish were fed 6 experimental diets containing different levels (2.19, 4.72, 7.18, 9.67, 12.21 and 14.69 g/kg, respectively) of methionine for 49 d. The results showed as follows: compared with the 2.19 g/kg methionine, increasing dietary methionine level significantly improved the weight gain, feed intake, feed efficiency, specific growth rate and protein efficiency ratio (P<0.05). When methionine level arrived at 7.18 g/kg, the weight gain and specific growth rate had the highest values. Methionine level had significant effects on hepatopancreas and intestine weight, hepatopancreas and intestine protein content and intestine length (P<0.05), but had no significant effects on intestosomatic index (P>0.05). When methionine level is greater than or equal to 4.72 g/kg, no significant differences were found in hepatopancreas and intestine weight and intestine length among all treatments (P>0.05). The hepatopancreas protein content and intestine protein content had the highest values when methionine level reached 9.67 and 12.21 g/kg, respectively. The activities of trypsin, chymotrypsin and lipase in intestine and hepatopancreas got the highest values when methionine level was up to 7.18 g/kg. The highest value of amylase activity in intestine were found in 4.72 g/kg methionine treatment, and there was significant effects between 4.72 g/kg methionine treatment and other treatments (P<0.05). The activities of Na+,K+-ATPase, alkaline phosphatase, γ-glutamyl transpeptidase and creatine kinase in each intestinal segment were significantly affected by methionine level (P<0.05). It is concluded that the optimal levels of dietary methionine can improve the growth performance, digestive and absorptive ability of grass carp during middle growth stage. Based on specific growth rate, the dietary methionine requirement of middle growth stage grass carp (259 to 498 g) is estimated to be 10.41 g/kg (34.71 g/kg crude protein) in the presence of 1.61 g/kg cystine.
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