Effects of Feeding Regimen and Dietary Tryptophan Level on Immune Function and Antioxidant Indices of Yangzhou Geese

WEI Zongyou; WANG Hongrong; PAN Xiaohua; YU Lihuai; JI Yun

doi:10.3969/j.issn.1006-267x.2012.12.010

Chinese Journal of Animal Nutrition >

2012 , Vol. 24 >Issue 12: 2356 - 2365

DOI: https://doi.org/10.3969/j.issn.1006-267x.2012.12.010

Swine and Poultry Nutrition

Effects of Feeding Regimen and Dietary Tryptophan Level on Immune Function and Antioxidant Indices of Yangzhou Geese

WEI Zongyou ,
WANG Hongrong ,
PAN Xiaohua ,
YU Lihuai ,
JI Yun

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1. College of Animal Science and Technology,Yangzhou University,Yangzhou 222500, China;
2. Taicang Animal Husbandry &. Veterinary Station, Taicang 215400, China

Received date: 2012-06-15

Online published: 2012-12-03

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Abstract

The objective of this study was investigate the effects of feeding regimen and dietary tryptophan level on immune function and antioxidant indices of Yangzhou geese. A total of 270 21-day-old Yangzhou geese were randomly divided into 6 groups with 3 replicates per group and 15 geese per replicate. The experiment lasted for 42 days. A 2?3 factorial design was adopted in this experiment, including two feeding regimen (ad libitum and feed restriction) and three dietary tryptophan levels (0.14%, 0.22% and 0.30%). The results showed as follows: 1) average daily feed intake (ADFI) and average daily gain (ADG) of geese in ad libitum group were significantly higher than those in feed restriction group (P<0.05), but feeding regimen had no significant effect on feed to gain ratio (P>0.05). Significant interaction was found between feeding regimen and tryptophan level in the ADFI and ADG of Yangzhou geese (P<0.05). 2) Feeding regimen had no significant effect on spleen index, thymus index and bursa of Fabricius index (P>0.05). Compared with low tryptophan level group, the spleen index of geese in high tryptophan level group was significantly increased (P<0.05), and dietary tryptophan level had no significant effect on thymus index and bursa of Fabricius index (P>0.05). 3) The serum immunoglobulin G (IgG) content, superoxide dismutase (SOD) activity and total antioxidant capacity (T-AOC) in middle and high tryptophan level groups were significantly higher than those in low tryptophan level group (P<0.05), and serum immunoglobulin M (IgM) in high tryptophan level group was significantly higher than that in low tryptophan level group (P<0.05), but dietary tryptophan level had no significant effect on serum immunoglobulin A (IgA) and malonaldehyde (MDA) contents and glutathione peroxidase (GSH-Px) activity (P>0.05). 4) Typical apoptosis was found in splenic lymphocyte of geese in low tryptophan level group by electron microscope, including cell deformation, nuclear chromatin condensation and coarctation, mitochondrial swelled and obscured, the appearance of bright area and cavitation in cristae, obvious degranulation and disorder of rough endoplasmic reticulum. In conclusion, adequate supplementation of tryptophan in basal diet can increase growth performance of geese. Geese fed diets with low tryptophan level lead to apoptosis of splenic lymphocyte, while tryptophan supplementation can ameliorate this circumstance, promote the development of the spleen, and improve the capacity of total antioxidant and humoral immunity.

Key words： dietary tryptophan level; feeding regimen; immunity; antioxidant

Cite this article

WEI Zongyou , WANG Hongrong , PAN Xiaohua , YU Lihuai , JI Yun . Effects of Feeding Regimen and Dietary Tryptophan Level on Immune Function and Antioxidant Indices of Yangzhou Geese[J]. Chinese Journal of Animal Nutrition, 2012 , 24(12) : 2356 -2365 . DOI: 10.3969/j.issn.1006-267x.2012.12.010

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